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首页> 外文期刊>Medicine and science in sports and exercise >Swimming training in rats increases cardiac MicroRNA-126 expression and angiogenesis
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Swimming training in rats increases cardiac MicroRNA-126 expression and angiogenesis

机译：大鼠游泳训练可增加心脏MicroRNA-126的表达和血管生成

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Purpose: MicroRNA (miRNA)-126 is angiogenic and has two validated targets: Sprouty-related protein 1 (Spred-1) and phosphoinositol-3 kinase regulatory subunit 2 (PI3KR2), negative regulators of angiogenesis by VEGF pathway inhibition. We investigated the role of swimming training on cardiac miRNA-126 expression related to angiogenesis. Methods: Female Wistar rats were assigned to three groups: sedentary (S), training 1 (T1, moderate volume), and training 2 (T2, high volume). T1 consisted of 60 min?d -1 of swimming, five times per week for 10 wk with 5% body overload. T2 consisted of the same protocol of T1 until the eighth week; in the ninth week, rats trained for two times a day, and in the 10th week, rats trained for three times a day. MiRNA and PI3KR2 gene expression analysis was performed by real-time polymerase chain reaction in heart muscle. We assessed markers of training, the cardiac capillary-fiber ratio, cardiac protein expression of VEGF, Spred-1, Raf-1/ERK 1/2, and PI3K/Akt/eNOS. Results: The cardiac capillary-fiber ratio increased in T1 (58%) and T2 (101%) compared with S. VEGF protein expression was increased 42% in T1 and 108% in T2. Cardiac miRNA-126 expression increased 26% (T1) and 42% (T2) compared with S, correlated with angiogenesis. The miRNA-126 target Spred-1 protein level decreased 41% (T1) and 39% (T2), which consequently favored an increase in angiogenic signaling pathway Raf-1/ERK 1/2. On the other hand, the gene expression of PI3KR2, the other miRNA-126 target, was reduced 39% (T1) and 78% (T2), and there was an increase in protein expression of components of the PI3K/Akt/eNOS signaling pathway in the trained groups. Conclusions: This study showed that aerobic training promotes an increase in the expression of miRNA-126 and that this may be related to exercise-induced cardiac angiogenesis, by indirect regulation of the VEGF pathway and direct regulation of its targets that converged in an increase in angiogenic pathways, such as MAPK and PI3K/Akt/eNOS.
机译：目的：MicroRNA（miRNA）-126具有血管生成作用，并具有两个经过验证的靶标：Sprouty相关蛋白1（Spred-1）和磷酸肌醇3激酶调节亚基2（PI3KR2），它们是通过VEGF途径抑制血管生成的负调节剂。我们调查了游泳训练对与血管新生相关的心脏miRNA-126表达的作用。方法：将雌性Wistar大鼠分为三组：久坐（S），训练1（T1，中量）和训练2（T2，高量）。 T1包括60分钟-1的游泳时间，每周10次，每周5次，身体超负荷5％。直到第8周为止，T2包含与T1相同的协议;在第九周，大鼠每天训练两次，在第10周，大鼠每天训练三次。通过实时聚合酶链反应在心肌中进行MiRNA和PI3KR2基因表达分析。我们评估了训练标志，心脏毛细纤维比率，VEGF，Spred-1，Raf-1 / ERK 1/2和PI3K / Akt / eNOS的心脏蛋白表达。结果：与S相比，T1（58％）和T2（101％）的心脏毛细纤维比率增加。VEGF蛋白表达在T1中增加42％，在T2中增加108％。与S相比，心脏的miRNA-126表达增加了26％（T1）和42％（T2），与血管生成有关。 miRNA-126靶标Spred-1蛋白水平降低了41％（T1）和39％（T2），因此有利于增加血管生成信号通路Raf-1 / ERK 1/2。另一方面，另一个miRNA-126靶标PI3KR2的基因表达降低了39％（T1）和78％（T2），并且PI3K / Akt / eNOS信号转导成分的蛋白质表达增加了。训练的小组中的途径。结论：这项研究表明，有氧训练可通过间接调节VEGF途径和直接调节其靶点而使miRNA-126的表达增加，而这可能与运动诱导的心脏血管生成有关。血管生成途径，例如MAPK和PI3K / Akt / eNOS。

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