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THE CRYSTAL STRUCTURE OF R(CCCCGGGG) IN TWO DISTINCT LATTICES

机译:两个离散晶格中R(CCCCGGGG)的晶体结构

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The structure of the RNA octamer r(C(4)G(4)) has been determined in two different crystal forms. The conformations of the RNA duplex in the rhombohedral and hexagonal lattices display only minimal deviations, demonstrating that the RNA double helix is considerably less deformable than the DNA double helix. For the first time the crystal structures of an A-RNA and an A-DNA with identical sequence can now be compared. The large number of ordered water molecules observed in the minor groove of the RNA duplex suggests that an important contribution to its higher rigidity derives from the improved hydration due to the presence of the 2'-hydroxyl groups. Our finding that the conformation of the RNA double helix is virtually unaffected by different crystal packing modes provides evidence that proteins may not alter the conformation of RNA stem regions in a significant way.
机译:RNA八聚体r(C(4)G(4))的结构已确定为两种不同的晶体形式。菱形和六边形晶格中RNA双链体的构象仅显示出最小的偏差,表明RNA双螺旋比DNA双螺旋的可变形性小得多。现在可以首次比较具有相同序列的A-RNA和A-DNA的晶体结构。在RNA双链体的小沟中观察到的大量有序水分子表明,由于2'-羟基的存在,改善了水合作用对提高其刚性具有重要作用。我们的发现RNA双螺旋的构象实际上不受不同晶体堆积模式的影响,这一发现提供了证据,表明蛋白质可能不会以显着方式改变RNA茎区域的构象。

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