5-Aza-2'-deoxycytidine is a potent inhibitor of DNA methyltransferase 3B and induces apoptosis in human endometrial cancer cell lines with the up-regulation of hMLH1.

Cui M; Wen Z; Chen J; Yang Z; Zhang H

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5-Aza-2'-deoxycytidine is a potent inhibitor of DNA methyltransferase 3B and induces apoptosis in human endometrial cancer cell lines with the up-regulation of hMLH1.

机译：5-Aza-2'-脱氧胞苷是一种有效的DNA甲基转移酶3B抑制剂，可通过hMLH1的上调诱导人子宫内膜癌细胞株的凋亡。

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The aim of our study was to evaluate the effects of 5-aza-2'-deoxycytidine (5-azadC) on cell growth inhibition, cell cycle arrest, apoptosis as well as the expression levels of hMLH1 and DNMT3B in human endometrial cancer cell lines. Ishikawa, HHUA, and KLE cell lines were used. After treatment with 5-azadC, cells were measured by MTT to detect the growth inhibition. Flow cytometry analysis was used to evaluate the cell cycle distribution and apoptosis effect. The expression of hMLH1 and DNMT3B was performed by real-time PCR and Western blotting analysis. The methylation status of the hMLH1 gene was monitored by methylation-specific PCR. We confirmed that 5-azadC treatment resulted in growth inhibition, G(2) arrest, and cell apoptosis in human endometrial cancer cell lines. Furthermore, the data obtained by real-time PCR and Western blotting analysis demonstrated that the expression of hMLH1 was up-regulated by 5-azadC treatment in Ishikawa cells, accompanied by down-regulation of DNMT3B expression, when 5-azadC led to cell inhibition, G(2)/M arrest, and apoptosis. Our results suggested that 5-azadC is a potent inhibitor of DNA methyltransferase 3B and induces apoptosis in Ishikawa cells with the up-regulation of hMLH1.

机译：我们的研究目的是评估5-氮杂-2'-脱氧胞苷（5-azadC）对人子宫内膜癌细胞系中细胞生长抑制，细胞周期阻滞，凋亡以及hMLH1和DNMT3B表达水平的影响。。使用了Ishikawa，HHUA和KLE细胞系。用5-azadC处理后，通过MTT测量细胞以检测生长抑制。流式细胞仪分析用于评估细胞周期分布和凋亡效应。 hMLH1和DNMT3B的表达通过实时PCR和Western印迹分析进行。通过甲基化特异性PCR监测hMLH1基因的甲基化状态。我们证实5-azadC治疗导致生长抑制，G（2）逮捕和人类子宫内膜癌细胞系中的细胞凋亡。此外，通过实时PCR和Western印迹分析获得的数据表明，当5-azadC导致细胞抑制时，石蜡细胞中5-azadC处理可上调hMLH1的表达，同时伴随DNMT3B表达下调。，G（2）/ M逮捕和凋亡。我们的研究结果表明5-azadC是一种有效的DNA甲基转移酶3B抑制剂，并通过hMLH1的上调诱导石川细胞凋亡。

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《Medical oncology》 |2010年第2期|共8页
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Cui M; Wen Z; Chen J; Yang Z; Zhang H;
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1. 5-Aza-2'-deoxycytidine is a potent inhibitor of DNA methyltransferase 3B and induces apoptosis in human endometrial cancer cell lines with the up-regulation of hMLH1. [J] . Cui M, Wen Z, Chen J, Medical oncology . 2010,第2期

机译：5-Aza-2'-脱氧胞苷是一种有效的DNA甲基转移酶3B抑制剂，可通过hMLH1的上调诱导人子宫内膜癌细胞株的凋亡。
2. Camptothecin-Induced Cell Proliferation Inhibition and Apoptosis Enhanced by DNA Methyltransferase Inhibitor, 5-Aza-2 '-deoxycytidine [J] . Ding L, Qiu L, Zhang JP, Biological & pharmaceutical bulletin . 2009,第6期

机译：DNA甲基转移酶抑制剂5-Aza-2'-脱氧胞苷增强喜树碱诱导的细胞增殖抑制和凋亡
3. Histone deacetylase inhibitors inducing human cervical cancer cell apoptosis by decreasing DNA-methyltransferase 3B [J] . LIU Ning, ZHAO Li-jun, LI Xiao-ping, 中华医学杂志（英文版） . 2012,第018期

机译：组蛋白脱乙酰基酶抑制剂通过降低DNA-甲基转移酶3B诱导人宫颈癌细胞凋亡
4. NCTD enhancing paclitaxel-induced apoptosis in liver cancer cells involving up-regulation of Bim [C] . Li Yan, Fang Fang, Chen Shuang, World Automation Congress . 2012

机译：NCTD增强紫杉醇诱导的肝癌细胞诱导涉及BIM的上调的肝癌细胞
5. Preclinical evaluation of 5-aza-2'-deoxycytidine, an inhibitor of DNA methylation, for therapy of breast cancer. [D] . Bovenzi, Veronica. 2001

机译：DNA甲基化抑制剂5-氮杂2'-脱氧胞苷的临床前评价，用于乳腺癌的治疗。
6. DNA Methylation Inhibitor 5-Aza-2′-Deoxycytidine Induces Reversible Genome-Wide DNA Damage That Is Distinctly Influenced by DNA Methyltransferases 1 and 3B [O] . Stela S. Palii, Beth O. Van Emburgh, Umesh T. Sankpal, 2008

机译：DNA甲基化抑制剂5-Aza-2-脱氧胞苷诱导可逆的全基因组DNA损伤该损伤明显受到DNA甲基转移酶1和3B的影响
7. Camptothecin-Induced Cell Proliferation Inhibition and Apoptosis Enhanced by DNA Methyltransferase Inhibitor, 5-Aza-2'-deoxycytidine [O] . Li Ding, Lei Qiu, Junping Zhang, 2009

机译：通过DNA甲基转移酶抑制剂，5-AZA-2'-脱氧胞苷增强CAMPTOTHENIN诱导的细胞增殖抑制和细胞凋亡

5-Aza-2'-deoxycytidine is a potent inhibitor of DNA methyltransferase 3B and induces apoptosis in human endometrial cancer cell lines with the up-regulation of hMLH1.

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