Distance and angular dependence of intensity ratios in laser-induced autofluorescence techniques.

Kwek LC; Fu S; Chia TC; Tang CL

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Distance and angular dependence of intensity ratios in laser-induced autofluorescence techniques.

机译：激光诱导的自发荧光技术中强度比的距离和角度依赖性。

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Laser-induced autofluorescence techniques have the potential to be used for the detection of preinvasive human cancer cells. For colorectal and gastrointestinal cancer cells, the light is introduced in vivo through endoscopic means and the probe tip is brought gently into contact with the tissue under investigation. However, it is often assumed that there is no distance or angular dependence in the intensity of the light collected from the probes. We performed an in vitro experiment in which we showed that there was indeed no angular dependence provided the angle of inclination of the probe to normal incidence is small. However, we find substantial fluctuation in the intensities of peaks for changing distances. These fluctuations can be eliminated by considering the ratio of the intensities from two spectral lines.

机译：激光诱导的自发荧光技术具有用于检测浸润前人类癌细胞的潜力。对于大肠癌细胞和胃肠道癌细胞，通过内窥镜将光引入体内，并使探针尖端与受研究的组织轻轻接触。然而，通常假设从探针收集的光的强度不存在距离或角度依赖性。我们进行了一项体外实验，在该实验中我们发现，只要探针相对于法线入射的倾斜角很小，就不会存在角度依赖性。但是，我们发现，随着距离的变化，峰值强度会发生很大的波动。通过考虑两条光谱线的强度比可以消除这些波动。

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《Medical Physics》 |2004年第5期|共4页
作者
Kwek LC; Fu S; Chia TC; Tang CL;
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原文格式 PDF
正文语种 eng
中图分类基础医学;
关键词
Methodology; Lasers; Light; Distance; in vivo; 激光; 光;

机译：Methodology;Lasers;Light;Distance;in vivo;激光;光;

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1. Distance and angular dependence of intensity ratios in laser-induced autofluorescence techniques. [J] . Kwek LC, Fu S, Chia TC, Medical Physics . 2004,第5期

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Distance and angular dependence of intensity ratios in laser-induced autofluorescence techniques.

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