Optical-resolution photoacoustic microscopy (OR-PAM) has been validated as a valuable tool for label-free volumetric microvascular imaging. More importantly, the advantages of noninvasiveness and measurement consistency suggest the use of OR-PAM for chronic imaging of intact microcirculation. Here, such chronic imaging is demonstrated for the first time by monitoring the healing process of laser-induced microvascular lesions in a small animal model in vivo. The central part of a 1 mm by 1 mm region in a nude mouse ear was treated under a continuous-wave laser to create a microvascular lesion for chronic study. The region of interest was imaged before the laser treatment, immediately after the treatment, and throughout the healing process using both the authors' OR-PAM system and a commercial transmission-mode optical microscope. Three-dimensional microvascular morphology and blood oxygenation information were imaged simultaneously at capillary-level resolution. Transmission-mode optical microscopic images were acquired for comparison. OR-PAM has potential important applications in microcirculatory physiology or pathophysiology, tumor angiogenesis, laser microsurgery, and neuroscience.
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机译：光学分辨率光声显微镜（OR-PAM）已被验证为无标签体积微血管成像的有价值的工具。更重要的是，无创性和测量一致性的优点表明使用OR-PAM进行完整微循环的慢性成像。在此，通过在体内小型动物模型中监测激光诱导的微血管病变的愈合过程，首次证明了这种慢性成像。在连续波激光下对裸鼠耳朵中1 mm x 1 mm区域的中央部分进行处理，以创建微血管病变，以进行长期研究。使用作者的OR-PAM系统和商用透射模式光学显微镜，在激光治疗之前，治疗之后以及整个愈合过程中对目标区域成像。三维微血管形态学和血液氧合信息同时以毛细管水平的分辨率成像。获取透射模式光学显微图像以进行比较。 OR-PAM在微循环生理或病理生理，肿瘤血管生成，激光显微外科和神经科学中具有潜在的重要应用。
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