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首页> 外文期刊>Medicinal and Aromatic Plant Science and Biotechnology >Antiproliferative and Antioxidant Effects of Three Hypericum Species of Turkish Origin: H. perforatum, H. montbretii and H. origanifolium
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Antiproliferative and Antioxidant Effects of Three Hypericum Species of Turkish Origin: H. perforatum, H. montbretii and H. origanifolium

机译：三种源自土耳其的金丝桃属植物的抗增殖和抗氧化作用：贯叶连翘，蒙氏双歧杆菌和折叶草

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摘要

Hypericum species have been used in traditional medicine for various purposes including neoplastic diseases. In the present study, antiproliferative and antioxidant activities of extracts of Hypericum perforatum, H. montbretii and H. origanifolium were investigated. Antiproliferative effects of Hypericum extracts were examined by using MTT method on A549 and HeLa cell lines. The beta-Carotenelinolenic acid system and the Rancimat method were used to evaluate antioxidant activities, while the DPPH method was employed for testing free radical scavenging activities of the extracts. Total phenolic compounds, flavonoids and flavonols were also measured, whereas major constituents like hypericin, hyperforin, quercetin, quercitrin, isoquercitrin, hyperoside, rutin and chlorogenic acid were determined by HPLC-DAD analyses. The findings indicated that flower extracts of H. montbretii and H. origanifolium have higher antioxidant and antiradical activities than the extract from aerial part of H. perforatum. While flower extract of H. origanifolium has no effect on MTT measurement of A549 cells, leaf extract of this plant at 250 mu g/ml concentration exhibits only a weak effect on these cells. Leaf and flower extracts of the same plant possess a similar activity with H. perforatum extract on MTT measurement of HeLa cells. As measured with MTT assay, flower extracts of H. montbretii at the concentrations of 100 and 250 mu g/ml exhibit an antiproliferative effects on A549 cells, which is in similar magnitude with H. perforatum extract. Leaf extract of H. montbretii seems to be ineffective on the proliferation of A549 cells, whereas it exhibit only a week antiproliferative effect on HeLa cells at 250 mu g/ml concentration. Results suggested that Hypericum species tested have antiproliferative and apoptotic effects, which seems to be related to their antioxidant activities.
机译：金丝桃属植物已经在传统医学中用于包括肿瘤疾病在内的各种目的。在本研究中，研究了贯叶连翘，蒙氏血吸虫和折叶H. origanifolium提取物的抗增殖和抗氧化活性。用MTT法检测了贯叶连翘提取物对A549和HeLa细胞株的抗增殖作用。 β-胡萝卜素亚油酸体系和Rancimat方法用于评估抗氧化活性，而DPPH方法用于测试提取物的自由基清除活性。还测量了总酚类化合物，类黄酮和类黄酮醇，而主要成分如金丝桃素，金丝桃素，槲皮素，槲皮素，异槲皮苷，超甙，芦丁和绿原酸均通过HPLC-DAD分析确定。这些发现表明，montbretii和origanifolium的花提取物比贯叶连翘地上部分的提取物具有更高的抗氧化和抗自由基活性。尽管原产H.origanifolium的花提取物对A549细胞的MTT测量没有影响，但该植物的叶提取物在250μg / ml的浓度下对这些细胞的作用却微弱。同一株植物的叶和花提取物在通入HeLa细胞的MTT测量中具有与贯叶连翘提取物相似的活性。如通过MTT测定法测量的，浓度为100和250μg/ ml的蒙氏嗜血杆菌的花提取物表现出对A549细胞的抗增殖作用，其与穿孔穿孔菌的提取物具有相似的程度。 montbretii的叶提取物似乎对A549细胞的增殖无效，而在250μg / ml的浓度下对HeLa细胞仅表现出一周的抗增殖作用。结果表明，测试的金丝桃属植物具有抗增殖和凋亡作用，这似乎与其抗氧化活性有关。

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