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首页> 外文期刊>Medicinal chemistry >Effect of polyphenolic compounds from Solanum torvum on plasma lipid peroxidation, superoxide anion and cytochrome P450 2E1 in human liver microsomes.
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Effect of polyphenolic compounds from Solanum torvum on plasma lipid peroxidation, superoxide anion and cytochrome P450 2E1 in human liver microsomes.

机译：龙葵多酚化合物对人肝微粒体血浆脂质过氧化，超氧阴离子和细胞色素P450 2E1的影响。

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摘要

Previous studies presented evidence that plants contain antioxidants that have free radical-scavenging properties. Overproduction of free radicals leads to oxidative stress, a factor associated with a variety of diseases, such as diabetes. Cytochrome P450 2E1 enzymes (CYP2E1) are involved in drug metabolism in the liver and metabolism of DNA-reaction generating intra-mitochondrial ROS, which leads to micro- and macro-vascular pathology in diabetes. Plant-based chemicals can affect CYP2E1 enzymes and related defense mechanisms, possibly leading to protection against oxidative stress. We investigated the effect of Solanum torvum (ST) extracts on the inhibition of CYP2E1 activity in human liver microsomes. ST extract was analyzed for antioxidant activity by the ABTS method. Polyphenolic compounds were measured by the total phenol content using the Folin-Ciocalteau reagent. Flavonoid and tannin content were analyzed by standard methods. Oxidative stress was evaluated by measuring lipid peroxidation by TBARS and superoxide anion scavenging levels in plasma from diabetic patients. Results showed that 10 mg/ml of ST had CYP2E1 catalytic inhibiting activity (57.16 %). The IC50 value of CYP2E1 catalytic inhibiting activity level was 5.14 mg/ml by concentration in a dependent manner. One gram of concentrated ST extract had an antioxidant activity index of 3.68 mg of trolox and 360.53 mg of ascorbic acid equivalent. Effects on free radical-scavenging, as measured by TBARS and superoxide anion, showed IC50 values of 20.60 and 10.26 microg/ml, respectively. Polyphenolic compounds found included phenol, flavonoid and tannin, measuring 160.30, 104.36 and 65.91 mg/g, respectively. These results imply that ST is a natural source of polyphenolic antioxidants, which have cytochrome P450 2E1 enzyme inhibiting and free radical scavenging properties, as related to lipid peroxidation and superoxide anion activity. ST could potentially be used for reducing oxidative stress in diabetes. Study for usage as a conventional medicine, including dosage and chronic toxicity in humans, should be pursued.
机译：先前的研究提供了植物含有抗氧化剂的证据，这些抗氧化剂具有清除自由基的特性。自由基的过度产生导致氧化应激，氧化应激与多种疾病（例如糖尿病）相关。细胞色素P450 2E1酶（CYP2E1）参与肝脏中的药物代谢和DNA反应的代谢，产生线粒体内的ROS，从而导致糖尿病的微血管和大血管病变。植物性化学物质可影响CYP2E1酶及相关防御机制，可能导致抗氧化应激。我们研究了茄提取物（ST）提取物对人肝微粒体中CYP2E1活性的抑制作用。通过ABTS方法分析ST提取物的抗氧化活性。使用Folin-Ciocalteau试剂通过总酚含量测量多酚化合物。黄酮和单宁含量通过标准方法进行分析。通过测量TBARS的脂质过氧化作用和糖尿病患者血浆中超氧阴离子清除水平来评估氧化应激。结果显示10 mg / ml的ST具有CYP2E1催化抑制活性（57.16％）。 CYP2E1催化抑制活性水平的IC50值为5.14 mg / ml。一克浓缩的ST提取物的抗氧化活性指数为3.68 mg trolox和360.53 mg抗坏血酸当量。用TBARS和超氧阴离子测得的清除自由基的作用，IC50值分别为20.60和10.26 microg / ml。发现的多酚化合物包括苯酚，类黄酮和单宁，含量分别为160.30、104.36和65.91 mg / g。这些结果暗示ST是多酚抗氧化剂的天然来源，其具有与脂质过氧化和超氧化物阴离子活性有关的细胞色素P450 2E1酶抑制和自由基清除特性。 ST可以潜在地用于降低糖尿病的氧化应激。应该研究用作常规药物的用途，包括对人的剂量和慢性毒性。

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