Detection of rpoB Mutations in Mycobacterium tuberculosis by Biprobe Analysis.

Edwards KJ; Metherell LA; Yates MSaunders NA

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Detection of rpoB Mutations in Mycobacterium tuberculosis by Biprobe Analysis.

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摘要

A biprobe assay utilizing LightCycler technology was developed to detect rifampin resistance-associated gene mutations in the Mycobacterium tuberculosis rpoB gene. Three biprobes detected all mutations present in the 46 rifampin-resistant isolates. Wild-type sequences were correctly identified in each case. The method was reproducible, accurate, and easy to use.

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《Journal of Clinical Microbiology》 |2001年第9期|3350-3352|共3页
作者
Edwards KJ; Metherell LA; Yates MSaunders NA;
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作者单位

Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, Colindale, London, NW9 5HT, United Kingdom.;

artners.org;

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原文格式 PDF
正文语种英语
中图分类医学微生物学（病原细菌学、病原微生物学）;
关键词
Mutation; Mycobacterium tuberculosis; 突变; 分支杆菌; 结核;

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Detection of rpoB Mutations in Mycobacterium tuberculosis by Biprobe Analysis.

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