(57) Abstract Use of genetics manner which is based on the fusion of the protein and alkaline phosphatase (Limetal., 1995) isolation of the new export protein of the human die tubercle bacillus was made possible. Long-cherished desire discloses the isolation of the gene which this export protein which first, is called DES the code is done, discloses also the distribution with the identification and various maikobakuteria interspecific simultaneously. Especially, the said protein the only, two iron of IIth class - shows the fact that it exists in that primary array amino acid which is discovered at level of the enzyme active site of the oxo protein family. In the protein of this family, the DES protein of the human die tubercle bacillus stearoyl ACP desachiyuraze does not show significant agreement. Antigen property of this protein was researched next. The DES protein of the human die tubercle bacillus was made to reveal excessively with the colon bacillus with rearrangement, protein die of this bacterial origin was refined next. The reactivity for the DES protein of the serum of the tubercular patient who is infected to the human die tubercle bacillus or the cow die tubercle bacillus was tested with western blotting. 100% of the patients whom it tests recognized this protein. It compared the serum of the patient who is ill other condition with a certain relation, strength of the antibody response for the DES protein which was measured by ELISA of the serum of the tubercular patient shows the fact that it is significant difference between strength of antibody response of the patient of these two categories. Therefore, as for the DES protein there is a possibility of being the means where the one for serum diagnosis of the tuberculosis is interesting.
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