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Antioxidant activity and chemical constituents of some Indonesian fruit peels

机译：一些印尼果皮的抗氧化活性和化学成分

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The ethanolic extracts and their fractions of some Indonesian fruit peels were screened for their free radical scavenging properties using vitamin-E as standard antioxidant. Free radical scavenging activity was evaluated using l,l-diphenyl-2-picrylhydrazyl (DPPH) free radical. The strongest antioxidant activity was shown by the Rambutan (Nephelium lappaceum Linn.), followed by Kelengkeng (Euphoria longan Lour. Steud) and Durian (Durio zibethinus Murr.) with IC50 values of 7.74, 11.85, and 28.83 (xg/mL, respectively comparable to vitamin-E(IC50 = 8.48 ± 0.1 ug/mL). The ethyl acetate fraction of these fruit peels extracts demonstrated the most active antioxidant activity compared with hexane, chloroform and methanol fractions. The chemical constituents of Rambutan and Durian were successfully isolated using vacuum liquid chromatography and radial chromatography technique, and these structures were characterizated based on the proton (1H) and carbon (13C) NMR spectra. The isolated compounds were ethyl gallate (1) from Rambutan (Nephelium lappaceum Linn.) rind, 4,4-dimethyl-poriferasta-18(19)-en-3-ol (2) and 3a-E-ferulyloxy-lup-20(29)-en-28-oic acid (3) from Durian (Durio zibethinus Murr.) peels. The results showed that prospective antioxidant contents in Rambutan's fruit peel extract was higher than Kelengkeng and Durian fruit peel extracts.
机译：使用维生素E作为标准抗氧化剂，筛选了印尼某些果皮的乙醇提取物及其馏分的自由基清除性能。使用1，1-二苯基-2-吡啶并肼基（DPPH）自由基评估自由基清除活性。红毛丹（Nephelium lappaceum Linn。），紧接着的Kelengkeng（Euphoria longan Lour。Steud）和Durian（Durio zibethinus Murr。）表现出最强的抗氧化活性，IC50值分别为7.74、11.85和28.83（xg / mL）。相当于维生素E（IC50 = 8.48±0.1 ug / mL），这些果皮提取物中的乙酸乙酯级分具有比己烷，氯仿和甲醇级分更高的抗氧化活性，已成功分离出红毛丹和榴莲的化学成分分别用质子（1H）和碳（13C）NMR光谱表征了这些结构，分离出的化合物为红毛丹（Nephelium lappaceum Linn。）的没食子酸乙酯（1），4，来自榴莲（Durio zibethinus Murr。）的4-二甲基-poriferasta-18（19）-en-3-ol（2）和3a-E-阿魏酰氧基lup-20（29）-en-28-oic acid（3）结果表明，红毛丹的预期抗氧化剂含量安氏的果皮提取物高于科伦肯和榴莲果皮提取物。

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