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Efficient and rapid in vitro regeneration of Ocimum americanum L. using nodal segments

机译：利用节节段高效快速地体外分离美洲大叶蝉

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摘要

An efficient and rapid plant regeneration protocol has been developed from nodal explants of Ocimum americanum L. of family Lamiaceae. Multiple shoot proliferation was initiated from nodal explants cultured on MS medium supplemented with various concentration of 6-benzyladenine (BA) (0.5-2.0 mg/1), kinetin (KN) (0.5-2.0 mg/1) and with combinations. For callus formation explants were cultured on MS medium with 2, 4-D (0.5-2.0 mg/1). Maximum numbers of shoots (11.66±0.27) with average length (4.96±0.02) were induced on medium containing BA (0.5 mg/1) + IAA (0.50 mg/1). Repeated subculture of in vitro proliferated shoots yielded 30-35 shoots from a single nodal segment explant after 4-5 months. Developed single shoots were transferred for rooting toVi MS medium containing IBA (1.0 mg/1) for best rooting (97.50%) of each shoot (5.08±0.15). The in vitro raised plantlets with well-developed shoots and roots were successfully established in garden soil, farm yard manure and sand (1:1:1) and were grownin green house with 80% survival rate.
机译：已经从唇形科的美洲Ocimum L.的结节外植体开发了有效且快速的植物再生方案。从在不同浓度的6-苄腺嘌呤（BA）（0.5-2.0 mg / 1），激动素（KN）（0.5-2.0 mg / 1）及其组合的MS培养基上培养的结节外植体开始多芽增殖。对于愈伤组织形成，将外植体在具有2、4-D（0.5-2.0mg / 1）的MS培养基上培养。在含有BA（0.5 mg / 1）+ IAA（0.50 mg / 1）的培养基上诱导出平均长度（4.96±0.02）的最大芽数（11.66±0.27）。在4-5个月后，对单个增生的节段外植体进行重复增殖的体外增殖芽继代培养，可得到30-35个芽。转移已发育的单芽以生根到含IBA（1.0 mg / 1）的Vi MS培养基上，以使每个芽最佳生根（97.50％）（5.08±0.15）。在花园土壤，农田肥料和沙子（1：1：1）上成功地建立了具有成熟芽和根的体外育苗，并在温室中生长，存活率达80％。

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