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In vitro propagation, qualitative phytochemical analysis and antioxidant activity of Ruta chalapensis L.

机译：小菜蛾的体外繁殖，定性植物化学分析和抗氧化活性

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摘要

In the present study, a protocol of micropropagation for Ruta chalapensis L. was established. Tissue culture studies of R. chalapensis was carried out to induce callus on MS basal medium supplemented with various hormones such as Indole-3-acetic acid(IAA), Indole-3-butyric acid (IBA), a-Naphthalene acetic acid (NAA) and 2,4-dichlorophenoxy acetic acid (2,4-D). Various explants, such as leaf, node and internode were used. The results showed that maximum callus production was achieved by using the hormone a-Naphthalene acetic acid (2.0 mg/ 1). Preliminary phytochemical screening of methanolic crude extract indicated the presence of alkaloids, carbohydrates, saponin and protein. Callus extract also showed the presence of alkaloids, carbohydrates and phenols. Phenol estimation of leaf and stem extracts showed 16.3 mg/g and 15.4 mg/g, respectively. Thin-layer chromatography was developed in a mixture of suitable solvent system (Hexane 70%: Ethyl acetate 30%). Methanolic extracts of leaf and stem showedgood antioxidant activity with IC50 value 96.9 tig/ml and 102.34xgl ml, respectively.
机译：在本研究中，建立了小菜蛾的微繁殖规程。进行了南美白对虾的组织培养研究，以MS培养基为基础诱导愈伤组织，MS培养基上添加了各种吲哚-3-乙酸（IAA），吲哚-3-丁酸（IBA），α-萘乙酸（NAA） ）和2,4-二氯苯氧基乙酸（2,4-D）。使用了各种外植体，例如叶，节点和节点间。结果表明，使用激素α-萘乙酸（2.0 mg / 1）可达到最大的愈伤组织产量。甲醇粗提物的初步植物化学筛选表明，存在生物碱，碳水化合物，皂苷和蛋白质。愈伤组织提取物还显示出生物碱，碳水化合物和酚的存在。叶和茎提取物的苯酚估计分别为16.3 mg / g和15.4 mg / g。在合适的溶剂系统（己烷70％：乙酸乙酯30％）的混合物中展开薄层色谱。叶和茎的甲醇提取物表现出良好的抗氧化活性，IC50值分别为96.9 tig / ml和102.34xgl ml。

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