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Polymorphism of heterochromatic regions of flax chromosomes.

机译:亚麻染色体异色区域的多态性。

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The C-banding technique was used to study flax chromosomes (Linum usitatissimum L., 2n = 30). Heterochromatin was located mainly in pericentromeric regions of chromosomes. In spite of small size (1.5-3.5 microm), all 15 pairs of homologous chromosomes were identified on the basis of the C-banding pattern and morphology. An idiogram of C-banded chromosomes of L usitatissimum L. is presented. Polymorphism of chromosomal heterochromatic regions was studied in karyotypes of three flax samples: L usitatissimum L., accession K-603 (L usitatissimum var. usitatissimum), and accession K-594 (L. usitatissimum var. humile (Mill.)). A common C-banding pattern was observed in all forms studied, although there were some distinctions in the individual band size. The fibre flax (accession K-603) karyotype had the C-banding pattern similar to that of L usitatissimum L., but some intercalary and telomeric C-bands were somewhat larger, and a satellite (NOR) was observed in the short arm of chromosome I. In crown flax, (K-594) chromosomal C-banding pattern exhibited smaller pericentromeric and larger intercalary bands; telomeric bands were present on almost all chromosomes. Thus, the intraspecies polymorphism revealed in the chromosomal C-banding pattern makes possible the use of C-bands as chromosome markers in the studies of genetic and genomic polymorphism of this species.
机译:C谱带技术用于研究亚麻染色体(Linum usitatissimum L.,2n = 30)。异染色质主要位于染色体的着丝粒区域。尽管体积小(1.5-3.5微米),但仍根据C带模式和形态鉴定了全部15对同源染色体。呈现了L usitatissimum L.的C带染色体的图谱。在三个亚麻样品的核型中研究了染色体异色区的多态性:L. usitatissimum L.,登录号K-603(L usitatissimum var。usitatissimum)和K-594(L. usitatissimum var。humile(Mill。))。在所有研究的形式中都观察到了常见的C谱带模式,尽管各个谱带大小存在一些差异。纤维亚麻(登录号K-603)核型的C带模式与L. usitatissimum L.相似,但有些cal间和端粒C带稍大,在其短臂上观察到卫星(NOR)。在冠亚麻中,(K-594)染色体C谱带模式显示较小的着丝粒和较大的cal带。几乎所有染色体上都存在端粒带。因此,染色体C带模式显示的种内多态性使得在研究该物种的遗传和基因组多态性中使用C带作为染色体标记成为可能。

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