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首页> 外文期刊>Membrane and cell biology >Participation of hyf-encoded hydrogenase 4 in molecular hydrogen release coupled with proton-potassium exchange in Escherichia coli.
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Participation of hyf-encoded hydrogenase 4 in molecular hydrogen release coupled with proton-potassium exchange in Escherichia coli.

机译：Hyf编码的加氢酶4参与分子氢释放以及大肠杆菌中的质子-钾交换。

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In a previous work (Trchounian et al., Biol. Membrany 16:416-428 (1999) (in Russian)) we reported the interrelations between production of H2 and H+-K+ exchange in fermenting Escherichia coli grown under anaerobic conditions at pH 7.5. The ion fluxes had stable stoichiometry 2H+/K+ and were N,N'-dicyclohexylcarbodiimide (DCC)-inhibitable at different external pH and K+ activity. In the present study, the H2 production was further studied in fermenting bacteria grown at pH 7.5 or 6.5. The H2 production was inhibited by DCC and did not occur if bacteria were grown at pH 7.5 in a medium containing formate or upon hypoosmotic stress. The H2 production was not sensitive to osmotic stress when bacteria were grown at pH 6.5. Formation of H2 and 2H+/K+ exchange were not observed in mutants with deletions of the hyfoperon genes, encoding membrane-associated hydrogenase 4. K+ influx in these mutants was not sensitive to valinomycin, in contrast to the K+ influx in the parental strain. If grown at pH 6.5, the mutants produced H2 and carried out 2H+/K+ exchange, when subjected to the hyperosmotic stress. The results suggest a participation of hydrogenase 4 in the production of H2 and proton-potassium exchange in fermenting E. coli grown at pH 7.5. In bacteria grown at pH 6.5 or in a medium containing formate, another membrane-bound hydrogenase, namely hydrogenase 3, may be responsible for the H2 production.
机译：在以前的工作中（Trchounian等人，Biol。Membrany 16：416-428（1999）（俄语）），我们报道了在pH 7.5的厌氧条件下生长的发酵大肠杆菌中H2的产生与H + -K +交换之间的相互关系。 。离子通量具有稳定的化学计量比2H + / K +，并且在不同的外部pH和K +活性下可抑制N，N'-二环己基碳二亚胺（DCC）。在本研究中，在pH 7.5或6.5的发酵细菌中进一步研究了H2的产生。 H2的产生受DCC抑制，如果细菌在含有甲酸盐的培养基中或在低渗胁迫下在pH 7.5下生长，则不会发生。当细菌在pH 6.5下生长时，H2的产生对渗透压不敏感。与编码膜相关的氢化酶4的hyfoperon基因缺失的突变体中，未观察到H2和2H + / K +交换的形成。与亲本菌株中的K +流入相反，这些突变体中的K +流入对valinomycin不敏感。如果在pH 6.5下生长，当受到高渗胁迫时，突变体会产生H2并进行2H + / K +交换。结果表明，在pH 7.5的发酵大肠杆菌中，氢酶4参与了H2的产生和质子-钾交换。在pH 6.5或含有甲酸盐的培养基中生长的细菌中，另一种与膜结合的氢化酶，即氢化酶3，可能负责产生H2。

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