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首页> 外文期刊>Membrane and cell biology >Activation of potassium channels in erythrocytes of marine teleost Scorpaena porcus.
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Activation of potassium channels in erythrocytes of marine teleost Scorpaena porcus.

机译：海洋硬骨蝎Scorpaena porcus红细胞中钾通道的激活。

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摘要

To assess the possibility of stimulating Ca2+-activated K+ channels, marine fish erythrocytes were incubated at 20-22 degrees C in saline containing a Ca2+-ATPase inhibitor (orthovanadate), a Ca2+ ionophore (A23187), propranolol or Pb2+. Incubation of the cells for up to 2 h under control conditions or in the presence of 5 mM NH4VO3 and 1 mM Ca2+ did not affect the intracellular K+ and Na+ concentrations. About 50% cellular K+ was lost from erythrocytes incubated in the presence of 0.01 mM A23187, 1 mM EGTA and 0.4-1.0 mM Ca2+. There was a significant loss of cellular K+ after the addition of 0.05-0.2 mM propranolol to the incubation medium. The stimulatory effect of propranolol on the K+ efflux was independent of external Ca2+. Blockers of Ca2+ transport, verapamil and Co2+, caused only a small decrease in the K+ loss induced by propranolol. The treatment of erythrocytes with 1-2 microM Pb2+ led to a minor K+ loss, but at a Pb2+ concentration of 20-50 microM, about 70% cellular K+ was lost. The K+ efflux induced by propranolol or Pb2+ was completely blocked by 1 mM quinine. The induced K+ loss from the erythrocytes was accompanied by a slight increase in the intracellular Na+ concentration. These data indicate the possibility of inducing Ca2+- and Pb2+-activated potassium channels in erythrocytes of S. porcus. A distinctive feature of the cells is a high sensitivity to propranolol, which activates K+ channels in the absence of external Ca2+.
机译：为了评估刺激Ca2 +激活的K +通道的可能性，将海水鱼类红细胞在20-22摄氏度下于含有Ca2 + -ATPase抑制剂（原钒酸盐），Ca2 +离子载体（A23187），普萘洛尔或Pb2 +的盐水中孵育。在对照条件下或在5 mM NH4VO3和1 mM Ca2 +存在下，将细胞孵育长达2小时不会影响细胞内K +和Na +的浓度。在0.01 mM A23187、1 mM EGTA和0.4-1.0 mM Ca2 +的存在下孵育的红细胞中约有50％的细胞K +丢失。向培养液中添加0.05-0.2 mM普萘洛尔后，细胞的K +大量损失。普萘洛尔对钾离子外流的刺激作用与外部钙离子无关。 Ca2 +转运，维拉帕米和Co2 +的阻滞剂仅引起普萘洛尔引起的K +损失的少量减少。用1-2 microM Pb2 +处理红细胞导致较小的K +损失，但是当Pb2 +浓度为20-50 microM时，约70％的细胞K +丢失。普萘洛尔或Pb2 +诱导的K +外排被1 mM奎宁完全阻断。红细胞诱导的K +丢失伴随着细胞内Na +浓度的轻微增加。这些数据表明可能在S.porcus的红细胞中诱导Ca 2+和Pb 2+激活的钾通道。细胞的一个显着特征是对普萘洛尔的高敏感性，在没有外部Ca2 +的情况下激活了K +通道。

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