• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Methods: A Companion to Methods in Enzymology >Emerging applications of flow cytometry in solid tumor biology
【24h】

Emerging applications of flow cytometry in solid tumor biology

机译：流式细胞术在实体肿瘤生物学中的新兴应用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Despite considerable interest during the early clinical development of flow cytometry, its application to solid tumours has been largely ignored in recent years. However, with rapid progress in cancer biology and molecular therapeutics, linked to technical developments in the areas of flow cytometry instrumentation, reagents, and data analysis, it is timely to re-evaluate this role. This article places emphasis on the unique potential of flow cytometry to analyze heterogeneous cell populations, and to provide information on the functional status of regulatory processes by the simultaneous measurement of multiple key elements. Major obstacles to progress addressed include the acquisition of adequate clinical samples, tissue disaggregation to produce single cells suspensions suitable for flow cytometry, and protocols to label intracellular as well as cell surface antigens.
机译：尽管在流式细胞术的早期临床开发过程中引起了极大的兴趣，但近年来它在实体瘤中的应用已被很大程度上忽略。但是，随着癌症生物学和分子治疗学的飞速发展，以及流式细胞仪，试剂和数据分析领域的技术发展，现在应该重新评估这种作用。本文重点介绍流式细胞仪分析异质细胞群体的独特潜力，并通过同时测量多个关键元素来提供有关调节过程功能状态的信息。解决进展的主要障碍包括获得足够的临床样品，组织分解产生适合流式细胞术的单细胞悬液，以及标记细胞内和细胞表面抗原的方案。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号