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首页> 外文期刊>Biochemistry >COUP-TF Plays a Dual Role in the Regulation of the Ovalbumin Gene
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COUP-TF Plays a Dual Role in the Regulation of the Ovalbumin Gene

机译：COUP-TF在卵清蛋白基因的调控中起着双重作用

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摘要

The ovalbumin (Ov) gene contains a number of regulatory elements that control its transcriptional activity and restrict expression to avian oviduct. One major regulatory region, the steroiddependent regulatory element (SDRE),is required for induction by estrogen and corticosterone. Another region, the negative regulatory element (NRE), downsteream of he SDRE, acts primarily to repress gene expression. In addition, experiments within indicate that the binding site for the COUP transcription factor (COUP-TF) is also required for Ov gene transcription. To examine the interactions involving the SDRE, the NRE,and the COUP binding sites on Ov gene transceription, mutations in these regions were made and transfected into primary oviduct cell cultures. These experiments shw that without the NRE, the SDRE is sufficient for induction by estrogen and corticosterone, irrespective of the COUP site.However, with the NRE intact, the COUP site is required for steroid induction,althought without the NRE, the COUP site attenuates transcriptional activity. More interestingly, overexpression of COUP-TF1 with the Ov wild-type reporter construct alleviates the requireement for steroid hormones. These results demonstrate that the COUP site is essential and has a dual role in Ov gene transcription and that steroid homones might directly or indirectly regulate the activity of COUP-TF1.
机译：卵清蛋白（Ov）基因包含许多调节元件，可控制其转录活性并将表达限制于禽输卵管。雌激素和皮质酮的诱导需要一个主要的调节区域，即类固醇依赖性调节元件（SDRE）。另一个区域，即负调控元件（NRE），即SDRE的下层，主要起抑制基因表达的作用。此外，内的实验表明，Ov基因转录也需要COUP转录因子（COUP-TF）的结合位点。为了检查涉及SDRE，NRE和Ov基因转录的COUP结合位点的相互作用，进行了这些区域的突变并将其转染到原代输卵管细胞培养物中。这些实验表明，没有NRE的情况下，SDRE足以被雌激素和皮质酮诱导，而与COUP位点无关。但是，在NRE完整的情况下，类固醇诱导需要COUP位点，即使没有NRE，COUP位点也会减弱。转录活性。更有趣的是，用Ov野生型报道基因构建物过度表达COUP-TF1可以减轻对类固醇激素的需求。这些结果表明，COUP位点是必不可少的，并且在Ov基因转录中具有双重作用，而类固醇同型激素可能直接或间接调节COUP-TF1的活性。

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