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Virulence markers of Helicobacter pylori in patients with diarrhoea-dominant irritable bowel syndrome

机译:腹泻型肠易激综合征患者中幽门螺杆菌的毒力标记

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摘要

Recent studies suggest that irritable bowel syndrome (IBS) is associated with low-grade inflammation. This study aims to determine the distribution of Helicobacter pylori cytotoxin-associated gene A {cagA) and vacuolating cytotoxin A (vacA) alleles (e.g., s1 and s2) in patients with diarrhoea-dominant IBS (IBS-D) as the latter causes vacuolation in colonic epithelial eels in vitro. One hundred and seventy patients meeting Rome HI criteria for IBS-D (mean age: 40 +-15 years) were enroled. Gastric biopsy was assessed histologieally and DNA extraction was performed by polymerase chain reaction (PCR) for H. pylori genus 16S ribosomal DMA (16S rDNA), cagA and vacA allele s1 and s2. There was no age- or gender-related difference in H, pylori positivity in IBS-D compared to the control group. H. pylori was positive in 116 (68%) with IBS-D compared to 88 (55%) in the control group
机译:最近的研究表明,肠易激综合症(IBS)与低度炎症有关。这项研究的目的是确定腹泻型IBS(IBS-D)患者中幽门螺杆菌细胞毒素相关基因A(cagA)和细胞毒素A(vacA)等位基因(例如s1和s2)的分布在体外结肠上皮鳗鱼中。入选符合IBS-D罗马HI标准(平均年龄:40 + -15岁)的170名患者。组织学评估胃活检,并通过聚合酶链反应(PCR)对幽门螺杆菌属16S核糖体DMA(16S rDNA),cagA和vacA等位基因s1和s2进行DNA提取。与对照组相比,IBS-D的H幽门螺杆菌阳性率与年龄或性别无关。 IBS-D的幽门螺杆菌阳性率为116(68%),而对照组为88(55%)(P <0.01)。 IBS-D的cagA阳性率为73(63%),而对照组为42(48%)(P = 0.03)。与IBS-D相比,vacA s1阳性的比例为61(53%),而对照组为32(36%)(P = 0.02)。 IBS-D的cagA s1阳性为39(34%),而对照组为13(15%)(P = 0.002)。

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