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首页> 外文期刊>Microbiology and Immunology >SEQUENCES OF E/NS1 GENE JUNCTION FROM FOUR DENGUE-2 VIRUSES OF NORTHEASTERN THAILAND AND THEIR EVOLUTIONARY RELATIONSHIPS WITH OTHER DENGUE-2 VIRUSES
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SEQUENCES OF E/NS1 GENE JUNCTION FROM FOUR DENGUE-2 VIRUSES OF NORTHEASTERN THAILAND AND THEIR EVOLUTIONARY RELATIONSHIPS WITH OTHER DENGUE-2 VIRUSES

机译：东北泰国的四种登革2型病毒E / NS1基因连接序列及其与其他登革2型病毒的进化关系

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摘要

We determined the 240-nucleotide sequences of the E/NS1 gene junction of four dengue-2 viruses by the primer extension dideoxy chain termination method. These viruses were isolated from dengue patients with different clinical severities in Nakhon Phanom, Northeastern Thailand in 1993. The results were compared with the 52 published dengue-2 sequences of the same gene region, Sequence divergence of four new isolates varied from 4.17 % to 5.42 % compared with dengue-2 prototype New Guinea C strain whereas it varied from 5.42% to 6.67% and from 6.67% to 7.09% when compared with Jamaica 1409 strain and PR159/S1 strain, respectively. All nucleotide substitutions were found at the 3rd position of the codons which were silent mutations. All 56 isolates studied were classified into five genotypic groups by constructing the dendrogram. The results indicated that four new isolates from Northeastern Thailand belong to genotype II of dengue virus serotype 2, and were most closely related to prototype New Guinea C strain. We also observed the variation in nucleotide and amino acid sequences among clusters of isolates (Thailand-1980, Malaysia-1989 and Thailand-1993) which were obtained from the dengue patients with different clinical severities. The significance of these genetic differences have been discussed in terms of the possible correlation between genetic variability and virulence.
机译：我们通过引物延伸双脱氧链终止法确定了四种登革2型病毒的E / NS1基因连接的240个核苷酸序列。这些病毒是从1993年在泰国东北那空帕侬市从具有不同临床严重程度的登革热患者中分离出来的。结果与公开的52个相同基因区域的登革热2序列进行了比较，四种新分离株的序列差异从4.17％至5.42不等。与登革2型原型新几内亚C菌株相比，其百分含量为％，而与牙买加1409菌株和PR159 / S1菌株相比，其分别为5.42％至6.67％和从6.67％至7.09％。在密码子的第3位发现所有核苷酸取代，它们是沉默突变。通过构建树状图，将研究的所有56个分离株分为5个基因型组。结果表明，来自泰国东北部的四个新分离株属于登革热病毒血清型2的基因型II，与原型新几内亚C菌株最相关。我们还观察到了从具有不同临床严重程度的登革热患者获得的分离株簇（泰国-1980，马来西亚-1989和泰国-1993）之间核苷酸和氨基酸序列的变化。就遗传变异性和毒力之间可能的相关性而言，已经讨论了这些遗传差异的重要性。

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