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首页> 外文期刊>Microbiology >Description of Anaerobacillus alkalilacustre gen. nov., sp. nov.—Strictly Anaerobic Diazotrophic Bacillus Isolated from Soda Lake and Transfer of Bacillus arseniciselenatis , Bacillus macyae, and Bacillus alkalidiazotrophicus to Anaerobacillus as the New Combinations A. arseniciselenatis comb. nov., A. macyae comb. nov., and A. alkalidiazotrophicus comb. nov
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Description of Anaerobacillus alkalilacustre gen. nov., sp. nov.—Strictly Anaerobic Diazotrophic Bacillus Isolated from Soda Lake and Transfer of Bacillus arseniciselenatis , Bacillus macyae, and Bacillus alkalidiazotrophicus to Anaerobacillus as the New Combinations A. arseniciselenatis comb. nov., A. macyae comb. nov., and A. alkalidiazotrophicus comb. nov

机译：碱性厌氧杆菌的描述。十一月，sp。十一月。严格地从苏打湖中分离出厌氧重氮芽孢杆菌，并将砷化芽孢杆菌，玛雅芽孢杆菌和碱性重氮芽孢杆菌转移到厌氧芽孢杆菌中，作为新的组合：A. arseniciselenatis梳。 11月，A。macyae梳子。十一月，和A.碱性重氮营养梳。十一月

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摘要

An anaerobic, spore-forming bacterium (strain Z-0521) was isolated from the iron-reducing microbial community enriched from sample of bottom sediments from low-mineralized soda lake Khadyn, Tuva upper Yenisey region (Russia). Cells of strain Z-0521 are motile straight Gram-positive rods, 0.7–1.1 ( μ m in diameter and 3.0–7.0 μ m length. It is a mesophilic halotolerante obligate alkaliphilic bacterium with a pH range for growth 8.5–10.7 (optimum at 9.6–9.7). Utilizes carbohydrates. Peptides, organic acids or alcohols are not utilized. In the presence of mannite strain Z-0521 reduces AQDS, arsenate, selenate and selenite. It is capable of N 2 fixation and has nitrogenase gene nif H. The dominant cellular fatty acids are C 16:0 , C 16:1w7c and C a15 . The G+C content in the DNA is 36.2 mol %. 16S rRNA gene sequencing identified strain Z-0521 as a member of rRNA group 6 of the genus Bacillus . Its closest relatives are B. alkalidiazotrophicus and B. macyae (98.3 and 98.1% sequence similarity). On the basis of physiological properties and genetic analysis, it is proposed that strain Z-0521 T should be assigned to a new species of a new genus, Anaerobacillus alkalilacustre gen. nov., sp. nov. It is also proposed that Bacillus arseniciselenatis , Bacillus macyae and Bacillus alkalidiazotrophicus should be transferred to this new genus, with Anaerobacillus arseniciselenatis (formely Bacillus arseniciselenatis ) as the type species.
机译：从富含矿物质的低铁苏打湖Khadyn底部叶状沉积物样品中分离出一种还原铁的微生物群落，分离出一种厌氧孢子形成细菌Z-0521（俄罗斯）。 Z-0521菌株的细胞是能动的，直的革兰氏阳性棒，直径为0.7–1.1（直径为μm，长度为3.0–7.0μm。这是嗜温的嗜盐嗜盐藻专性嗜碱细菌，pH范围可生长8.5–10.7（最佳于9.6–9.7）。利用碳水化合物，不使用肽，有机酸或醇类，在甘露聚糖菌株Z-0521的存在下，AQDS，砷酸盐，硒酸盐和亚硒酸盐都能被还原，它能够被N 2固定，并具有N酶基因nifH。主要的细胞脂肪酸为C 16：0，C 16：1w7c和C a15，DNA中G + C含量为36.2 mol％，16S rRNA基因测序鉴定出Z-0521菌株为该rRNA组6的成员。芽孢杆菌属，其近亲是碱性重氮芽孢杆菌和Macyae杆菌（序列相似性分别为98.3和98.1％），根据生理特性和遗传分析，建议将Z-0521 T菌株分配给一个新的还提出了一个新属，碱厌氧厌氧杆菌属，nov。，sp.nov。应将砷化芽孢杆菌，Macyae芽孢杆菌和碱性重氮芽孢杆菌转移到这个新属中，并以厌氧芽孢杆菌（以前称为砷化芽孢杆菌）作为类型种。

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