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首页> 外文期刊>Microbiology and Immunology >Evaluation of chimeric DNA vaccines consisting of premembrane and envelope genes of Japanese encephalitis and dengue viruses as a strategy for reducing induction of dengue virus infection-enhancing antibody response
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Evaluation of chimeric DNA vaccines consisting of premembrane and envelope genes of Japanese encephalitis and dengue viruses as a strategy for reducing induction of dengue virus infection-enhancing antibody response

机译：评价由日本脑炎和登革热病毒的前膜和包膜基因组成的嵌合DNA疫苗，作为减少诱导登革热病毒感染的抗体反应的策略

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摘要

Neutralizing antibodies induced by dengue virus (DENV) infection show viral infection-enhancing activities at sub-neutralizing doses. On the other hand, preimmunity against Japanese encephalitis virus (JEV), a congener of DENV, does not increase the severity of DENV infection. Several studies have demonstrated that neutralizing epitopes in the genus Flavivirus are mainly located in domain III (DIII) of the envelope (E) protein. In this study, chimeric premembrane and envelope (prM-E) gene-based expression plasmids of JEV and DENV1 with DIII substitution of each virus were constructed for use as DNA vaccines and their immunogenicity evaluated. Sera from C3H/He and ICR mice immunized with a chimeric gene containing DENV1 DIII on a JEV prM-E gene backbone showed high neutralizing antibody titers with less DENV infection-enhancing activity. Our results confirm the applicability of this approach as a new dengue vaccine development strategy.
机译：登革病毒（DENV）感染诱导的中和抗体在亚中和剂量下显示出增强病毒感染的活性。另一方面，针对日本脑炎病毒（DENV的同类病毒）的免疫前接种不会增加DENV感染的严重性。数项研究表明，黄病毒属中的表位主要位于包膜（E）蛋白的结构域III（DIII）中。在这项研究中，构建了以JEV和DENV1嵌合体前膜和包膜（prM-E）基因为基础的表达质粒，每种病毒都被DIII取代，用作DNA疫苗并评估了它们的免疫原性。在JEV prM-E基因主链上用含有DENV1 DIII的嵌合基因免疫的C3H / He和ICR小鼠的血清显示出高中和抗体滴度，而DENV感染增强活性较低。我们的结果证实了这种方法作为新型登革热疫苗开发策略的适用性。

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