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首页> 外文期刊>Microbial Pathogenesis >Presence of a functional nitrate assimilation pathway in Mycobacterium smegmatis
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Presence of a functional nitrate assimilation pathway in Mycobacterium smegmatis

机译：耻垢分枝杆菌中功能性硝酸盐同化途径的存在

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Ability of Mycobacterium smegmatis to assimilate nitrate was evaluated in its active and dormant phase. Nitrate (10mM), nitrite (0.5mM) and ammonia (10mM) allowed growth of M. smegmatis concomitant with their complete depletion from the culture in 144, 120 and 96h, respectively, when used as sole nitrogen source. Azide (50muM) stopped the growth of M. smegmatis when nitrate was used as sole nitrogen source. l-methionine-S-sulfoximine (l-MSO), which is a well-known inhibitor of glutamine synthetase, an enzyme also involved in nitrogen metabolic pathway, when applied at 10mug/ml concentration, completely inhibited the growth of the organism when nitrate or nitrite was used as sole nitrogen source. There was no effect of either azide or l-MSO at above concentrations on the growth of the organism when asparagine or ammonia was used as sole nitrogen source. More significantly, utilization of nitrate, nitrite and ammonia continued even in oxygen depletion induced dormant culture at the rates of 289, 25 and 354muM/day, respectively. These rates were 5-8 times slower than the rates of 1966, 127 and 2890muM/day, respectively, in active replicating phase. In the presence of azide (50muM) and l-MSO (10mug/ml), 2.1 and 1.51 logs reduction in viability of dormant M. smegmatis was observed using nitrate and nitrite, respectively, as sole nitrogen source. Altogether, the results indicated the presence of nitrate assimilation pathway operating in both active and dormant stage of M. smegmatis.
机译：评价了耻垢分枝杆菌吸收硝酸盐的活跃期和休眠期的能力。硝酸盐（10mM），亚硝酸盐（0.5mM）和氨水（10mM）分别在144、120和96h内使耻垢分枝杆菌生长并完全耗尽，同时用作唯一的氮源。当硝酸盐用作唯一的氮源时，叠氮化物（50μM）阻止了耻垢分枝杆菌的生长。 l-甲硫氨酸-S-磺胺嘧啶（l-MSO）是众所周知的谷氨酰胺合成酶抑制剂，谷氨酰胺合成酶也是一种参与氮代谢途径的酶，当以10mug / ml的浓度施用时，当硝酸盐被完全抑制时，生物体的生长受到抑制或亚硝酸盐用作唯一的氮源。当天冬酰胺或氨用作唯一的氮源时，以上浓度的叠氮化物或1-MSO对生物体的生长没有影响。更重要的是，即使在耗氧引起的休眠培养中，硝酸盐，亚硝酸盐和氨的利用率仍在继续，分别为289、25和354muM / day。在主动复制阶段，这些速率分别比1966、127和2890μM/ day的速率慢5-8倍。在叠氮化物（50μM）和1-MSO（10μg/ ml）的存在下，分别使用硝酸盐和亚硝酸盐作为唯一的氮源，观察到潜伏的耻垢分枝杆菌的活力降低了2.1和1.51log。总之，结果表明在耻垢分枝杆菌的活跃和休眠阶段都存在硝酸盐同化途径。

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