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首页> 外文期刊>Microbial Pathogenesis >Differential regulation of cytokine genes in gingival epithelial cells challenged by Fusobacterium nucleatum and Porphyromonas gingivalis
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Differential regulation of cytokine genes in gingival epithelial cells challenged by Fusobacterium nucleatum and Porphyromonas gingivalis

机译：核梭菌和牙龈卟啉单胞菌攻击的牙龈上皮细胞中细胞因子基因的差异调节

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摘要

IL-8 mRNA in human gingival epithelial cells (HGECs) is up-regulated by Fusobacterium nucleatum, and up-/down-regulated by Porphyromonas gingivalis in a complex interaction in the early stages (ltoreq 4 h) after infection. The mechanisms involved in this regulation in response to F. nucleatum and/or P. gingivalis infection, and identification of co-regulated cytokine genes, are the focus of this investigation. Heat, formalin or protease treatment of F. nucleatum cells attenuated the IL-8 mRNA up-regulation. NF-kappaB, mitogen-activated protein kinase (MAPK) p38 and MAPK kinase/extracellular signal-regulated kinase (MEK/ERK) pathways were involved in IL-8 mRNA induction by F. nucleatum. Pretreatment of P. gingivalis with heat, formalin or protease enhanced IL-8 mRNA induction. NF-kappaB, MARK p38, and MEK/ERK pathways were also involved in this induction. In contrast, down-regulation of IL-8 mRNA by P. gingivalis involved MEK/ERK, but not NF-kappaB or MAPK p38 pathways. cDNA arrays analysis revealed that mRNA down-regulation by P. gingivalis is a specific reaction that only a number of genes, e.g. IL-1beta, IL-8, macrophage inflammatory protein-2alpha, and migration inhibitory factor-related protein-14, are affected based on examination of 278 cytokine/receptor genes. These data indicate that F. nucleation and P. gingivalis trigger specific and differential gene regulation pathways in HGECs. Copyright 2005 Elsevier Ltd. All rights reserved.
机译：在感染后的早期（ltoreq 4 h）中，人牙龈上皮细胞（HGECs）中的IL-8 mRNA在复杂的相互作用中被核梭状芽孢杆菌上调，并被牙龈卟啉单胞菌上/下调。本研究的重点是响应于核仁镰刀菌和/或牙龈卟啉单胞菌感染而参与该调节的机制，以及鉴定共同调节的细胞因子基因。热，福尔马林或蛋白酶处理的核仁镰刀菌细胞减弱了IL-8 mRNA的上调。 NF-κB，丝裂原活化蛋白激酶（MAPK）p38和MAPK激酶/细胞外信号调节激酶（MEK / ERK）途径参与了F. nucleatum诱导的IL-8 mRNA的表达。用热，福尔马林或蛋白酶预处理牙龈卟啉单胞菌可增强IL-8 mRNA的诱导。 NF-κB，MARK p38和MEK / ERK途径也参与了这种诱导。相反，牙龈卟啉单胞菌对IL-8 mRNA的下调涉及MEK / ERK，但不涉及NF-κB或MAPK p38途径。 cDNA阵列分析表明，牙龈卟啉单胞菌的mRNA下调是一种特异反应，它只有许多基因，例如大肠杆菌。根据对278个细胞因子/受体基因的检查，IL-1beta，IL-8，巨噬细胞炎性蛋白2alpha和迁移抑制因子相关蛋白14受到影响。这些数据表明F.成核和牙龈卟啉单胞菌触发HGECs中的特定和差异基因调控途径。版权所有2005 ElsevierLtd。保留所有权利。

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