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首页> 外文期刊>Memoirs of the Queensland Museum >A preliminary investigation of the utility of ribosomalgenes for species identification of Sea Anemones(Cnidaria: Actiniaria)
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A preliminary investigation of the utility of ribosomalgenes for species identification of Sea Anemones(Cnidaria: Actiniaria)

机译：核糖体基因在海葵物种识别中的实用性的初步研究

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摘要

The utility of the ribosomal DNA gene complex for species identification of Actiniaria wasexamined. The use of universal ribosomal PCR primers is problematic in this group due tothe presence of algal symbionts. Universal primers were initially used to amplify a regioncontaining partial 18S, complete ITS, 5.8S, ITS2, and partial 28S sequences from sixsea anemone species. The development of two sea anemone specific primers for thisregion was necessary to avoid amplification of algal symbionts for a number of species.Complete sequences of the 18S-28S fragment were obtained from three species,?Anemonia sp. (724 bp), Heteractis malu (670 bp) and Stichodactyla haddoni (734 bp);partial or non-overlapping sequences were obtained from Entacmaea quadricolor(480bp from 18S), Macrodactyla doreensis (523 bp: 300bp from 18S and 223bp from28S) and Oulactis muscosa (556bp: 285bp from 18s and 271bp from 28S). Averagesequence divergence among sea anemone species was approx. 24% indicating that thisregion may indeed be useful for species identification. However, unexpectedly lowdivergence recorded between two species in different genera, neither of which could beverified by histology due to specimen unavailability, indicated that traditional histologicalmethods are still needed to confirm identification and certainly until such time that anrDNA database of sea anemone tissue has been established.
机译：研究了核糖体DNA基因复合物在猕猴桃属鉴定中的应用。由于藻类共生体的存在，在该组中通用核糖体PCR引物的使用存在问题。通用引物最初用于扩增包含六海葵物种的部分18S，完整ITS，5.8S，ITS2和28S部分序列的区域。为避免许多物种的藻类共生体扩增，必须为该区域开发两个海葵特异性引物。从三个物种——Anemonia sp。获得了18S-28S片段的完整序列。 （724 bp），马氏杂种优势（670 bp）和哈氏Stichodactyla haddoni（734 bp）;部分或不重叠序列分别从四足Entacmaea（从18S获得480bp），Macrodactyla doreensis（523 bp：从18S获得300bp和从28S获得223bp）和粘壁油ul（Oulactis muscosa）（556bp：18s起285bp，28s起271bp）。海葵物种之间的平均序列差异约为。 24％的人表示该地区确实可能对物种识别有用。然而，在不同属的两个物种之间记录的出乎意料的低差异性，由于样本不可用而无法通过组织学进行验证，这表明仍然需要传统的组织学方法来确认识别，并且肯定要等到建立海葵组织的anrDNA数据库为止。

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