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首页> 外文期刊>Memoirs of the Queensland Museum >The use of infrared spectroscopy as a test forspecies-specific pedal mucus in gastropod molluscs— a comparative study in Moreton Bay and Singapore
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The use of infrared spectroscopy as a test forspecies-specific pedal mucus in gastropod molluscs— a comparative study in Moreton Bay and Singapore

机译：红外光谱法在腹足动物软体动物中检测特定种类踏板黏液的研究—在摩顿湾和新加坡进行的比较研究

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Infrared (IR) spectroscopy was used to study pedal mucous characteristics of eight speciesof marine gastropods to determine if it was useful for species identification. Three speciesof Nerita, two morphologically indistinguishable species of Littoraria (L. articulate and L.strigata) and one species each of Nodilittorina, Austrolittorina and Echinolittorina, werecompared with a terrestrial snail, Achatina fulica. The IR spectra all showed high absorptionin the 900-2100 cm 1 wave number region. No benzene overtones were observed at lowwave numbers (1800-2000 cm 1) suggesting that aromatic amino acids could be lackingin core glycoprotein molecules. Strong absorption bands at peaks of 1644 cm 1 and1545 cm 1 were obtained for all species and attributable to amide-I and amide-II peaksrespectively. Amide I (range 1600-1720 cm 1) to Amide II (1500-1600 cm-1) band arearatios ranged significantly: Nerita species: 1: 0.10-1: 0.22; Achatina fulica 1: 0.40; andLittoraria sp. 1: 0.48; Austrolittorina unifastciata: 1: 0.57; Echinolittorina malaccana: 1: 0.65and Nodilittorina pyramidalis: 1: 0.74. These differences appear useful for distinguishingbetween the six genera and congeneric species studied.
机译：红外（IR）光谱用于研究八种海洋腹足动物的踏板粘液特性，以确定其是否对物种识别有用。比较了三种Nerita物种，两种形态上难以区分的Littoraria物种（L. articulate和L.strigata）以及Nodilittorina，Austrolittorina和Echinolittorina的一种，与陆地蜗牛Achatina fulica进行了比较。红外光谱在900-2100 cm 1波数区域均显示出高吸收。在低波数（1800-2000 cm 1）下未观察到苯的泛音，表明核心糖蛋白分子中可能缺少芳香族氨基酸。对于所有物种，在1644 cm 1和1545 cm 1的峰处均获得了强吸收带，这分别归因于酰胺I和酰胺II峰。酰胺I（16000-1720 cm 1的范围）至酰胺II（1500-1600 cm-1）的带面积比显着变化：Nerita物种：1：0.10-1：0.22; Achatina fulica 1：0.40;和立陶宛1：0.48;南方fast：1：0.57;疟原虫：1：0.65和Nodilittorina pyramidalis：1：0.74。这些差异似乎有助于区分所研究的六个属和同类属。

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