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首页> 外文期刊>Microbial Pathogenesis >Enteroaggregative Escherichia coli (EAEC) strains enter and survive within cultured intestinal epithelial cells
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Enteroaggregative Escherichia coli (EAEC) strains enter and survive within cultured intestinal epithelial cells

机译：肠聚合性大肠杆菌（EAEC）菌株进入培养的肠上皮细胞并在其中生存

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Enteroaggregative Escherichia coli (EAEC) is an emerging pathogen associated to cases of acute or persistent diarrhea in children and adults from developed and developing countries. These microorganisms also have been isolated from human immunodeficiency virus-infected patients. EAEC exhibits aggregative adherence (AA) in HEp-2 cells. This pattern is characterized by the production of bacteria aggregates adhered to monolayer cultured cells with a ''stacked brick'' phenotype. The AA pattern is related to the presence of a 60MDa plasmid (pAA). In the present study, we evaluated the adherence, invasion and persistent survival of five EAEC strains with Caco-2 and T84 cells, by a bacteria invasion assay and transmission electron microscopy. EAEC isolated from cases of acute infantile diarrhea can be internalized by intestinal epithelial cells cultivated in vitro, suggesting that these strains may employ a mechanism of host cell invasion to colonize the intestinal mucosa. Results showed that EAEC strains could survive intracellularly up to 72h. Our data support evidence that EAEC is able to invade, persist and replicate within intestinal cells for extended time. This strategy may be advantageous to EAEC in colonization and survival, favoring the exploitation of an intracellular niche where these strains are protected against host clearance mechanisms, immune system and antibiotic treatment. Intracellular persistence of EAEC may be associated with development of persistent diarrhea associated to thesemicroorganisms. To our knowledge, this is the first report of EAEC intracellular survival in cultured intestinal epithelial cells.
机译：肠聚合性大肠杆菌（EAEC）是一种新兴病原体，与发达国家和发展中国家的儿童和成人的急性或持续性腹泻有关。这些微生物也已经从感染人类免疫缺陷病毒的患者中分离出来。 EAEC在HEp-2细胞中表现出聚集粘附（AA）。这种模式的特征是产生附着在单层培养细胞上的具有“堆积砖”表型的细菌聚集体。 AA模式与60MDa质粒（pAA）的存在有关。在本研究中，我们通过细菌入侵试验和透射电子显微镜评估了五种EAEC菌株与Caco-2和T84细胞的粘附，侵袭和持续存活。从急性小儿腹泻病例中分离出的EAEC可以通过体外培养的肠上皮细胞内化，这表明这些菌株可能利用宿主细胞入侵的机制定居在肠粘膜中。结果表明，EAEC菌株可以在细胞内存活72小时。我们的数据支持了EAEC能够在肠道细胞内长时间侵入，持久和复制的证据。这种策略可能对EAEC的定殖和生存有利，有利于利用细胞内的生态位，这些菌株可以保护宿主免受宿主清除机制，免疫系统和抗生素治疗的侵害。 EAEC的细胞内持久性可能与这些微生物相关的持续性腹泻的发展有关。据我们所知，这是在培养的肠上皮细胞中EAEC细胞内存活的首次报道。

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