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首页> 外文期刊>Microbial Pathogenesis >Development of a conjugate vaccine against invasive pneumococcal disease based on capsular polysaccharides coupled with PspA/family 1 protein of Streptococcus pneumoniae
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Development of a conjugate vaccine against invasive pneumococcal disease based on capsular polysaccharides coupled with PspA/family 1 protein of Streptococcus pneumoniae

机译：基于荚膜多糖与肺炎链球菌PspA /家族1蛋白结合的抗侵袭性肺炎球菌疾病的结合疫苗的开发

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The efforts were focused on exploring alternative pneumococcal vaccine strategies, aimed at addressing the shortcomings of existing formulations, without compromising efficacy. Our strategy involved the use of the carrier protein, pneumococcal surface protein A (PspA), conjugated with capsular polysaccharides (CPS), to provide effective and non-serotype-dependent protection. In this study, we generated a stable Escherichia coli construct expressing functional PspA from a capsular serotype 6B strain and confirmed it belonging to family I, which was conjugated with CPS. The distribution of anti-CPS antibody response was almost completely of IgG2a subclass followed by IgG3 and low level of IgG1 subclass, but that of anti-PspA IgG subclass antibodies was almost equal IgG1 and IgG2a subclasses. Though PspA was less conspicuous on the surface of pneumococci than the capsule, the antibodies induced with CPS-rPspA conjugate possessed more accessibility to the surface of Streptococcus pneumoniae serotype 6B and 19F (the same family 1 PspA). By survival experiment, the result suggested that the level of cross-protection after immunized with the conjugate was more measurable within the same family I. The CPS-rPspA conjugate not only induced CPS-specific protection but also provided PspA specific cross-protection. (c) 2015 Elsevier Ltd. All rights reserved.
机译：努力的重点是探索其他肺炎球菌疫苗策略，旨在解决现有制剂的缺点，而又不影响功效。我们的策略涉及使用与荚膜多糖（CPS）偶联的载体蛋白，肺炎球菌表面蛋白A（PspA），以提供有效且非血清型依赖性的保护。在这项研究中，我们从荚膜血清型6B菌株中生成了一个稳定的表达功能性PspA的大肠杆菌构建体，并证实它属于与CPS偶联的家族I。抗CPS抗体反应的分布几乎完全是IgG2a亚类，其次是IgG3和低水平的IgG1亚类，但是抗PspA IgG亚类抗体的分布几乎等同于IgG1和IgG2a亚类。尽管PspA在肺炎球菌表面上不如在胶囊中显着，但CPS-rPspA共轭物诱导的抗体对肺炎链球菌血清型6B和19F（同一家族1 PspA）的可及性更高。通过存活实验，结果表明用缀合物免疫后的交叉保护水平在同一家族I中更可测量。CPS-rPspA缀合物不仅诱导了CPS特异性保护，而且提供了PspA特异性交叉保护。 （c）2015 Elsevier Ltd.保留所有权利。

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