• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Microbial Pathogenesis >Membrane sorting during swimming internalization of Brucella is required for phagosome trafficking decisions
【24h】

Membrane sorting during swimming internalization of Brucella is required for phagosome trafficking decisions

机译：布鲁氏菌的游泳内在化过程中进行膜分选是吞噬体运输决定的必要条件

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Brucella infects macrophages by swimming internalization, after which it is enclosed in macropinosomes. We investigated the role of the uptake pathway in phagosome trafficking, which remains unclear. This study found membrane sorting during swimming internalization and is essential in intracellular replication of Brucella. The B. abortus virB mutant replicated intracellularly when it was in the macropinosome established by wild-type B. abortus that retained its ability to alter phagosome trafficking. Lipid rafts-associated molecules, such as GM1 ganglioside, were selectively included into macropinosomes, but Rab5 effector early endosome autoantigen (EEA1) and lysosomal glycoprotein LAMP-1 were excluded from macropinosomes containing B. abortus induced by swimming internalization. In contrast, when the swimming internalization was bypassed by phorbol myristate acetate (PMA)-induced macropinocytosis, lipid raft-associated molecules were excluded, and EEA1 and LAMP-1 were included into macropinosomes containing bacteria. The phosphatidylinositol 3-kinase inhibitor wortmannin that inhibits PMA-induced macropinocytosis blocked internalization of virB mutant, but not of wild-type of B. abortus and wortmannin treatment did not affect intracellular replication. Our results suggest that membrane sorting requires swimming internalization of B. abortus and decides the intracellular fate of the bacterium, and that Brucella-induced macropinosome formation is a different mechanism from PMA-induced macropinocytosis.
机译：布鲁氏菌通过游泳内在化作用感染巨噬细胞，然后被巨噬细胞包裹。我们调查了摄取途径在吞噬体运输中的作用，目前尚不清楚。这项研究发现了在游泳内在化过程中的膜分选，对于布鲁氏菌的细胞内复制至关重要。流产双歧杆菌virB突变体位于野生型流产双歧杆菌所建立的巨细胞体中时，可在细胞内复制，并保持其改变吞噬体运输的能力。脂筏相关分子，例如GM1神经节苷脂，被选择性地包含在大胶体中，但是Rab5效应物早期内体自身抗原（EEA1）和溶酶体糖蛋白LAMP-1被排除在由游泳内在化引起的流产双歧杆菌的大胶体中。相反，当游泳内在化作用被佛波肉豆蔻酸酯乙酸酯（PMA）诱导的巨胞饮作用绕开时，脂质筏相关分子被排除在外，而EEA1和LAMP-1被包含在含有细菌的巨胞体中。抑制PMA诱导的巨胞饮作用的磷脂酰肌醇3激酶抑制剂渥曼青霉素阻止了virB突变的内在化，但野生型双歧杆菌和渥曼青霉素的处理并未影响细胞内复制。我们的结果表明，膜分选需要流产双歧杆菌的内在化并决定细菌的细胞内命运，布鲁氏菌诱导的巨胞体形成与PMA诱导的巨胞饮作用不同。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号