...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry >Identification and characterization of functional cation coordination sites in platelet endothelial cell adhesion molecule-1
【24h】

Identification and characterization of functional cation coordination sites in platelet endothelial cell adhesion molecule-1

机译：血小板内皮细胞粘附分子-1中功能性阳离子配位点的鉴定与表征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We have employed 45CaCl2 binding studies, terbium (Tb3+) luminescence spectroscopy, and electrospray mass spectroscopy (ESI-MS) to identify divalent metal binding properties of soluble recombinant human PECAM-1 (srPECAM-1), and to define unique cation binding domains using short, linear peptide sequences from the protein. PECAM-1 was found to directly interact with 45CaCl2, binding 2.3 nmol of Ca2+mol of srPECAM-1 with a Kd of 1.17 nM. PECAM-1 was found to contain high-affinity cation binding sites involving amino acids Asp443, Asp444, and Glu446 of Ig-domain 5 and residues Glu487, Glu490, Asp491, Glu538, Glu540, and Glu542 of Ig-domain 6. The PECAM cation binding sites demonstrated broad specificity for all divalent cations, with Mn2+ having a higher affinity than Ca2+ or Mg2+. Direct binding of Tb3+ to these PECAM peptides was confirmed by ESI-MS. Modeling studies predict that the six cation binding residues within Ig-domain 6 are proximal to each other in three-dimensional space, and may form a single cation coordination site. The identification of cation binding sites in PECAM-1 will direct further work in examining its cation-dependent roles in cellular signaling.
机译：我们已经进行了45CaCl2结合研究，ter（Tb3 +）发光光谱和电喷雾质谱（ESI-MS）来鉴定可溶性重组人PECAM-1（srPECAM-1）的二价金属结合特性，并使用以下方法定义独特的阳离子结合域来自蛋白质的短的线性肽序列。发现PECAM-1直接与45CaCl2相互作用，以1.17 nM的Kd结合2.3 nmol的Ca2 + / nmol的srPECAM-1。发现PECAM-1包含涉及Ig域5的氨基酸Asp443，Asp444和Glu446以及Ig域6的残基Glu487，Glu490，Asp491，Glu538，Glu540和Glu542的高亲和力阳离子结合位点。结合位点对所有二价阳离子显示出广泛的特异性，其中Mn2 +的亲和力高于Ca2 +或Mg2 +。通过ESI-MS证实了Tb3 +与这些PECAM肽的直接结合。建模研究预测，Ig结构域6中的六个阳离子结合残基在三维空间中彼此接近，并且可能形成单个阳离子配位位点。 PECAM-1中阳离子结合位点的鉴定将指导进一步研究其在细胞信号传导中的阳离子依赖性作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号