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首页> 外文期刊>Mikrochimica Acta: An International Journal for Physical and Chemical Methods of Analysis >Antibody-free colorimetric determination of total aflatoxins by mercury(II)-mediated aggregation of lysine-functionalized gold nanoparticles
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Antibody-free colorimetric determination of total aflatoxins by mercury(II)-mediated aggregation of lysine-functionalized gold nanoparticles

机译：汞（II）介导的赖氨酸官能化金纳米颗粒的聚集体的无抗体比色法测定总黄曲霉毒素

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摘要

Currently known immunoassays for aflatoxins (AFs) usually are not capable of simultaneous determination of total AFs (i.e., aflatoxin B-1, aflatoxin B-2, aflatoxin G(1), aflatoxin G(2)) due to the lack of group-specific antibodies. We are presenting here a colorimetric method for determination of total AFs by exploiting the Hg(II) ion-mediated aggregation of lysine-functionalized gold nanoparticles (Lys-AuNPs). AFs bind Hg(II) ion to form stable complexes, and this prevents the Hg(II) induced aggregation of Lys-AuNPs. Hence, the color change of solutions of AuNPs that occurs after aggregation of the AuNPs is suppressed. This effect was exploited to design a method for quantitation of AFs that can be detected by UV-Vis spectrophotometry (by measuring the ratio of absorbances at 725 nm and 525 nm), and even with bare eyes. The method has a very low detection limit (1.1 ppb) and is highly selective for AFs over other mycotoxins. Colorimetric analysis of rice samples by this method gave results that were in good agreement with those obtained by HPLC. To the best of our knowledge, this is the first method for visual detection of total AFs based on the distance-dependent optical properties of AuNPs.
机译：由于缺乏组别，目前已知的黄曲霉毒素（AFs）免疫分析通常无法同时测定总AFs（即黄曲霉毒素B-1，黄曲霉毒素B-2，黄曲霉毒素G（1），黄曲霉毒素G（2））。特异性抗体。我们在这里介绍一种比色法，通过利用Hg（II）离子介导的赖氨酸功能化金纳米颗粒（Lys-AuNPs）的聚集来确定总AF。 AFs与Hg（II）离子结合形成稳定的复合物，从而阻止了Hg（II）诱导的Lys-AuNPs聚集。因此，抑制了在AuNP的聚集之后发生的AuNP的溶液的颜色变化。利用这种效果设计了一种定量AF的方法，该方法可以通过UV-Vis分光光度法（通过测量725 nm和525 nm的吸光度之比）甚至裸眼来检测。该方法的检出限非常低（1.1 ppb），对AF的选择性比其他霉菌毒素高。用这种方法对大米样品进行比色分析，得出的结果与通过HPLC获得的结果非常吻合。据我们所知，这是基于AuNPs随距离变化的光学特性进行总AF视觉检测的第一种方法。

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