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首页> 外文期刊>Mikrochimica Acta: An International Journal for Physical and Chemical Methods of Analysis >Application of polypyrrole/GOx film to glucose biosensor based on electrochemical-surface plasmon resonance technique
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Application of polypyrrole/GOx film to glucose biosensor based on electrochemical-surface plasmon resonance technique

机译：基于表面电化学等离子体共振技术的聚吡咯/ GOx膜在葡萄糖生物传感器中的应用

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摘要

A surface plasmon resonance (SPR) based biosensor for glucose is presented in which a thin gold film modified with polypyrrole and glucose oxidase (PPy-GOx) acts as the sensor chip. It is based on SPR response to the change of refractive index of PPy-GOx film by the enzymatic catalytic reaction. The co-electropolymerization of pyrrole and GOx was carried out under cyclic voltammetric conditions, and simultaneously monitored by in-situ SPR. It has been revealed that the enzymatic reaction between GOx and PPy in the presence of glucose can lead to distinct changes in the SPR signal. From the experiments, a linear relationship was obtained in the range 1-100 μmol L~(-1) between glucose concentration and the rate of redox transformation of PPy. The detection limit was 0.5 μmol L~(-1) (S/N=3) and recoveries were 95.2-102.7%.
机译：提出了一种基于表面等离振子共振（SPR）的葡萄糖生物传感器，其中用聚吡咯和葡萄糖氧化酶（PPy-GOx）修饰的金薄膜充当传感器芯片。它基于SPR通过酶催化反应对PPy-GOx薄膜折射率变化的响应。吡咯和GOx的共电聚合是在循环伏安条件下进行的，同时通过原位SPR进行监测。已经发现在葡萄糖存在下GOx和PPy之间的酶促反应可以导致SPR信号的明显变化。实验表明，葡萄糖浓度与PPy的氧化还原转化率呈线性关系，范围为1-100μmolL〜（-1）。检出限为0.5μmolL〜（-1）（S / N = 3），回收率为95.2-102.7％。

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