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首页> 外文期刊>Biochemistry >Non-Enzymatic Interaction of Reaction Products and Substrates in Peroxidase Catalysis
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Non-Enzymatic Interaction of Reaction Products and Substrates in Peroxidase Catalysis

机译：过氧化物酶催化中反应产物和底物的非酶相互作用

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摘要

A quantitative approach for estmation of the non-enzymatic interaction between ammonium 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate)(ABTS)oxidation product and a poory oxidized substrate was developed using a system including tobacco peroxidase,a mediator substrate (ABTS),and a second substrate.The approach is based on the establishment of a pseudo-steady-state concentration of the ABTS oxidation product in the course of co-oxidation with a poor substrate.A mathematical description of the experimental curve shape has been proposed to linearize the kinetic data and estimate the rate constant for such non-enzymatic interaction.The rate constants calculated from the steady-state kinetics for the non-enzymatic interacton of ABTS oxidation product with phenol and resorcinol were 360+-40 and 770+-60 M~(-1)(centre dot)sec~(-1),respectively.The values obained have teh same order of magnitude as the rate constant for ABTS oxidation product interaction with veratryl alochol,calculated from electrochemical measurements (170 M~(-1)(centre dot)sec~(-1))by Donal Leech's group.However,the kinetic curves for co-oxidation of ABTS and veratryl alcohol catalyzed by tobacco peroxidase exhibit a pronounced lagperiod,which either points to the high rate of the non-enzymatic interaction between ABTS oxidation product and veratryl alcohol and thus,contradicts the electrocemical calculations,or indicates an enzymatic nature of the co-oxidation phenomenon in this particular case.
机译：利用包括烟草过氧化物酶的系统，开发了一种定量方法，用于估算2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸盐）（ABTS）氧化产物与弱氧化底物之间的非酶相互作用。介体底物（ABTS）和第二种底物。该方法基于在与不良底物共氧化过程中建立ABTS氧化产物的拟稳态浓度。实验曲线的数学描述已经提出了形状以线性化动力学数据并估计这种非酶相互作用的速率常数。从ABTS氧化产物与苯酚和间苯二酚的非酶相互作用的稳态动力学计算的速率常数为360 + -40和770 + -60 M〜（-1）（中心点）sec〜（-1）。得出的值与ABTS氧化产物与藜芦醇甲酚的相互作用的速率常数的数量级相同，由Donal Leech等人的电化学测量（170 M〜（-1）（中心点）sec〜（-1））。但是，烟草过氧化物酶催化的ABTS和藜芦醇共氧化的动力学曲线显示出明显的滞后性，要么表明ABTS氧化产物与藜芦醇之间非酶相互作用的发生率很高，从而矛盾了电化学计算，要么表明在这种特殊情况下共氧化现象的酶性质。

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