Expression,Isoltion,Purification,and Biochemical Properties of Trehalose-6-phosphate Hdrolase fom Thermoresistant Strain Bacillus sp.GP16

d.v.Karelov; V.V.Krasikov; M.A.Surjik; L.M.Firsov

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Expression,Isoltion,Purification,and Biochemical Properties of Trehalose-6-phosphate Hdrolase fom Thermoresistant Strain Bacillus sp.GP16

机译：耐热菌株芽孢杆菌GP16海藻糖6-磷酸水解酶的表达，分离，纯化及生化特性

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Here we describe cloning,expression,adn purification of the enzyme trehalose-6-phosphate hdyrolase from thermoresistant strain Bacillus sp.GP16.Principal biochemical properties of the enzyme at different pH and temperature values were determined.Entropy and enthalpy of activation of the enzyme for substrates trehalose-6-phosphate and p-nitrophenyl glucoside were calculated,and the dependence of the kinetic parameters from ionic strength was established.

机译：在此我们描述了耐热菌株Bacillus sp.GP16的海藻糖6-磷酸海藻糖酶的克隆，表达和adn的纯化。确定了该酶在不同pH和温度下的主要生化特性。计算了6-磷酸海藻糖和对硝基苯基葡萄糖苷的底物，建立了动力学参数对离子强度的依赖性。

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《Biochemistry》 |2003年第9期|共8页
作者
d.v.Karelov; V.V.Krasikov; M.A.Surjik; L.M.Firsov;
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正文语种 eng
中图分类生物化学;
关键词
trehalose-6-phosphate hydrolase; cloning of a gene; gene expression; catalysis; temperature dependence; determination of Michaelis constants;

机译：海藻糖6-磷酸水解酶;基因克隆;基因表达;催化;温度依赖性;Michaelis常数的测定;

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1. Expression,Isoltion,Purification,and Biochemical Properties of Trehalose-6-phosphate Hdrolase fom Thermoresistant Strain Bacillus sp.GP16 [J] . d.v.Karelov, V.V.Krasikov, M.A.Surjik, Biochemistry . 2003,第9期

机译：耐热菌株芽孢杆菌GP16海藻糖6-磷酸水解酶的表达，分离，纯化及生化特性
2. Expression, purification, and biochemical properties of arginase from Bacillus subtilis 168 [J] . Yu J.-J., Park K.-B., Kim S.-G., The journal of microbiology . 2013,第2期

机译：枯草芽孢杆菌168精氨酸酶的表达，纯化和生化特性
3. Cloning of aminoglycoside phosphotransferase (APH) gene from antibiotic-producing strain of Bacillus circulans into a high-expression vector, pKK223-3. Purification, properties and location of the enzyme [J] . The biochemical journal . 1991,第3期

机译：将产自环生芽孢杆菌的抗生素菌株的氨基糖苷磷酸转移酶（APH）基因克隆到高表达载体pKK223-3中。酶的纯化，性质和位置
4. Recombinant Expression and Biochemical Characterization of Levansucrase from Halophilic Bacteria Bacillus licheniformis BK1 and BK2 [C] . Nur Umriani Permatasari, Enny Ratnaningsih, Rukman Hertadi International Conference on the Frontier in Molecular Sciences . 2021

机译：来自嗜盐菌芽孢杆菌的重组表达和生物化学表征嗜盐菌芽孢杆菌Baceniformis BK1和BK2
5. Overexpression, purification, and characterization of AcpK, PksG, and MmgA from Bacillus subtilis strain 168. [D] . Williams, Jayme. 2007

机译：来自枯草芽孢杆菌菌株168的AcpK，PksG和MmgA的过表达，纯化和表征。
6. Cloning of aminoglycoside phosphotransferase (APH) gene from antibiotic-producing strain of Bacillus circulans into a high-expression vector pKK223-3. Purification properties and location of the enzyme [O] . 1991

机译：从生产抗生素的环状芽孢杆菌菌株中将氨基糖苷磷酸转移酶（APH）基因克隆到高表达载体pKK223-3中。酶的纯化性质和位置
7. Cloning of aminoglycoside phosphotransferase (APH) gene from antibiotic-producing strain of Bacillus circulans into a high-expression vector, pKK223-3. Purification, properties and location of the enzyme [O] . 1991

机译：从生产抗生素的环状芽孢杆菌菌株中将氨基糖苷磷酸转移酶（APH）基因克隆到高表达载体pKK223-3中。酶的纯化，性质和位置

Expression,Isoltion,Purification,and Biochemical Properties of Trehalose-6-phosphate Hdrolase fom Thermoresistant Strain Bacillus sp.GP16

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