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首页> 外文期刊>Microscopy research and technique >Oral tissue response to ovine grafting biomaterial: Morphological and morphometric study using scanning electron and light microscopy tissue response to ovine grafting biomaterial
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Oral tissue response to ovine grafting biomaterial: Morphological and morphometric study using scanning electron and light microscopy tissue response to ovine grafting biomaterial

机译:口腔组织对绵羊移植生物材料的反应:使用扫描电子和光学显微镜组织对绵羊移植生物材料的反应的形态学和形态计量学研究

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Objective: To evaluate the oral tissue response to an experimental particle ovine biomaterial by scanning electron microscopy (SEM) and light microscopy (LM). Material And Methods: Forty-eight rats had surgical periodontal defects treated with either blood clotting (control), bovine biomaterial? (B), or an experimental ovine biomaterial (O). Data from SEM analysis (defect exposure, root surface exposure, diameter of matrix fibers and bundles, and globuli areas; n = 5) were applied to Shapiro-Wilk, Kruskal-Wallis, and Dunn's test, whereas LM analysis (tissue cicatrization characteristics and diameter defect; n = 3) had data applied to two-way analysis of variance. Animals were monitored for 1 and 3 weeks. RESULTS: By SEM, the O samples showed significant differences from B and C in the area of defect exposure (H _(2,15) = 8.66; P < 0.05). In both periods, O and B samples showed similar results for matrix fiber diameters, differently than C samples (H _(2,15) = 14.0; P < 0.05). All other SEM variables were considered equivalent among the groups (P > 0.05). Under LM, an acute and chronic granulomatous inflammation was seen in the presence of both biomaterials (B and O, 1 week); both the control and the ovine grafting samples showed mature bone in the repair site (3 weeks); the defect diameter showed similar values among groups, at both monitoring periods (F _(2,12) = 1.0401; P > 0.05). Conclusion: The ovine particles of this study showed a favorable response to oral tissue repair, demonstrating to be a potential source for the development of bone grafting biomaterials.
机译:目的:通过扫描电子显微镜(SEM)和光学显微镜(LM)评估口腔组织对实验性颗粒绵羊生物材料的反应。材料和方法:48只大鼠患有外科牙周缺损,可以通过凝血(对照),牛生物材料治疗? (B)或实验性绵羊生物材料(O)。来自SEM分析的数据(缺陷暴露,根部表面暴露,基质纤维和束的直径以及球状区域; n = 5)应用于Shapiro-Wilk,Kruskal-Wallis和Dunn's检验,而LM分析(组织骨化特性和直径缺陷; n = 3)将数据应用于方差的双向分析。监测动物1和3周。结果:通过SEM,O样品在缺陷暴露区域上与B和C有显着差异(H _(2,15)= 8.66; P <0.05)。在两个时期中,O和B样品对基体纤维直径显示出相似的结果,与C样品不同(H _(2,15)= 14.0; P <0.05)。所有其他SEM变量在各组之间均视为等效(P> 0.05)。在LM下,两种生物材料均存在急性和慢性肉芽肿性炎症(B和O,1周)。对照和羊移植样品在修复部位均显示成熟的骨(3周);在两个监测期间,缺陷直径在各组之间显示相似的值(F _(2,12)= 1.0401; P> 0.05)。结论:这项研究的绵羊颗粒对口腔组织修复显示出良好的反应,证明是开发骨移植生物材料的潜在来源。

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