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首页> 外文期刊>Mechanisms of Ageing and Development >Telomerase activity in hybrids between telomerase-negative and telomerase-positive immortal human cells is repressed in the different complementation groups but not in the same complementation group of immortality.
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Telomerase activity in hybrids between telomerase-negative and telomerase-positive immortal human cells is repressed in the different complementation groups but not in the same complementation group of immortality.

机译：端粒酶阴性和端粒酶阳性永生人类细胞之间的杂交体中的端粒酶活性在永生的不同互补组中受到抑制，但在相同的永生性互补组中则没有。

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摘要

The expression of telomerase is essential for cells to be immortalized, and most immortal cell lines possessed telomerase activity. Using the cell fusion technique, it has been shown that mortal and telomerase-negative phenotypes of normal cells are dominant over immortal and telomerase-positive phenotypes, suggesting that the normal cells possessed dominant repressor-type activity for telomerase expression. Several telomerase-negative immortal human cell lines were reported, in which telomerase-independent mechanisms was supposed to maintain telomere length. We aimed at seeing whether the telomerase-negative phenotype of these immortal cells is dominant over telomerase-positive phenotype of other immortal cells in correlation with cellular mortality. Results showed that, when telomerase-positive and -negative immortal parental cell lines belonging to the different complementation groups were fused, telomerase-negative mortal hybrid clones arose, i.e. telomerase-negative phenotype was dominant as well as mortal phenotype. However, when immortal hybrid cells arose from telomerase-positive and -negative immortal parents belonging to either the same or different complementation groups, they were all telomerase-positive, i.e. telomerase-negative phenotype appeared to be recessive. Telomerase-negative immortal hybrid was never established from any combinations between telomerase-negative and -positive immortal parental cells.
机译：端粒酶的表达对于细胞永生化至关重要，大多数永生细胞系都具有端粒酶活性。使用细胞融合技术，已显示正常细胞的凡人和端粒酶阴性表型比不朽和端粒酶阳性表型占优势，这表明正常细胞具有端粒酶表达的显性阻遏物型活性。报道了几种端粒酶阴性的永生人类细胞系，其中端粒酶非依赖性机制被认为可以维持端粒长度。我们旨在观察这些永生细胞的端粒酶阴性表型是否与其他永生细胞的端粒酶阳性表型相关，并且与细胞死亡率相关。结果表明，当融合属于不同互补组的端粒酶阳性和阴性的永生亲代细胞系融合时，端粒酶阴性的凡人杂种克隆出现，即端粒酶阴性的表型和凡人的表型都占优势。但是，当来自属于相同或不同互补组的端粒酶阳性和阴性永生父母的永生杂种细胞出现时，它们都是端粒酶阳性的，即端粒酶阴性表型似乎是隐性的。端粒酶阴性和阳性永生亲代细胞之间的任何组合都从未建立端粒酶阴性永生杂种。

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