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首页> 外文期刊>Mechanisms of Development >A targeted genetic screen identifies crucial players in the specification of the Drosophila abdominal Capaergic neurons
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A targeted genetic screen identifies crucial players in the specification of the Drosophila abdominal Capaergic neurons

机译：有针对性的遗传筛查可确定果蝇腹部Capaergic神经元的规格中的关键参与者

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The central nervous system contains a wide variety of neuronal subclasses generated by neural progenitors. The achievement of a unique neural fate is the consequence of a sequence of early and increasingly restricted regulatory events, which culminates in the expression of a specific genetic combinatorial code that confers individual characteristics to the differentiated cell. How the earlier regulatory events influence post-mitotic cell fate decisions is beginning to be understood in the Drosophila NB 5-6 lineage. However, it remains unknown to what extent these events operate in other lineages. To better understand this issue, we have used a very highly specific marker that identifies a small subset of abdominal cells expressing the Drosophila neuropeptide Capa: the ABCA neurons. Our data support the birth of the ABCA neurons from NB 5-3 in a cas temporal window in the abdominal segments A2-A4. Moreover, we show that the ABCA neuron has an ABCA-sibling cell which dies by apoptosis. Surprisingly, both cells are also generated in the abdominal segments A5-A7, although they undergo apoptosis before expressing Capa. In addition, we have performed a targeted genetic screen to identify players involved in ABCA specification. We have found that the ABCA fate requires zfh2, grain, Grunge and hedgehog genes. Finally, we show that the NB 5-3 generates other subtype of Capa-expressing cells (SECAs) in the third suboesophageal segment, which are born during a pdm/cas temporal window, and have different genetic requirements for their specification
机译：中枢神经系统包含由神经祖细胞产生的各种神经元亚类。独特的神经命运的实现是一系列早期和日益受限的调节事件的结果，最终导致特定遗传组合密码表达的产生，该密码将个体特征赋予分化的细胞。在果蝇NB 5-6谱系中已经开始理解早期的调控事件如何影响有丝分裂后细胞的命运决定。但是，仍不清楚这些事件在其他谱系中发挥到何种程度。为了更好地理解这个问题，我们使用了非常高特异性的标记，可以识别一小部分表达果蝇神经肽Capa的腹部细胞：ABCA神经元。我们的数据支持NB 5-3的ABCA神经元在腹部节段A2-A4的cas颞窗中的诞生。此外，我们显示ABCA神经元有一个ABCA兄弟细胞，该细胞通过凋亡而死亡。出人意料的是，尽管它们在表达Capa之前会发生凋亡，但在腹部A5-A7中也都产生了这两种细胞。此外，我们进行了有针对性的遗传筛选，以识别参与ABCA规范的参与者。我们发现，ABCA的命运需要zfh2，谷物，Grunge和刺猬基因。最后，我们表明NB 5-3在食管下第三个节段中产生了Capa表达细胞（SECA）的其他亚型，它们在pdm / cas时间窗期间出生，并且对其规格有不同的遗传要求

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