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首页> 外文期刊>Mechanisms of Development >Innexin2 gap junctions in somatic support cells are required for cyst formation and for egg chamber formation in Drosophila.
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Innexin2 gap junctions in somatic support cells are required for cyst formation and for egg chamber formation in Drosophila.

机译：果蝇中的囊肿形成和卵室形成需要体细胞支持细胞中的Innexin2间隙连接。

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摘要

Germ cells require intimate associations with surrounding somatic cells during gametogenesis. During oogenesis, gap junctions mediate communication between germ cells and somatic support cells. However, the molecular mechanisms by which gap junctions regulate the developmental processes during oogenesis are poorly understood. We have identified a female sterile allele of innexin2 (inx2), which encodes a gap junction protein in Drosophila. In females bearing this inx2 allele, cyst formation and egg chamber formation are impaired. In wild-type germaria, Inx2 is strongly expressed in escort cells and follicle cells, both of which make close contact with germline cells. We show that inx2 function in germarial somatic cells is required for the survival of early germ cells and promotes cyst formation, probably downstream of EGFR pathway, and that inx2 function in follicle cells promotes egg chamber formation through the regulation of DE-cadherin and Bazooka (Baz) at the boundary between germ cells and follicle cells. Furthermore, genetic experiments demonstrate that inx2 interacts with the zero population growth (zpg) gene, which encodes a germline-specific gap junction protein. These results indicate a multifunctional role for Inx2 gap junctions in somatic support cells in the regulation of early germ cell survival, cyst formation and egg chamber formation. Inx2 gap junctions may mediate the transfer of nutrients and signal molecules between germ cells and somatic support cells, as well as play a role in the regulation of cell adhesion. Copyright Copyright 2011 Elsevier Ireland Ltd. All rights reserved.
机译：生殖细胞在配子发生过程中需要与周围的体细胞密切联系。在卵子发生过程中，间隙连接介导生殖细胞和体细胞支持细胞之间的通讯。然而，人们对卵裂在产卵过程中调节发育过程的分子机制了解甚少。我们已经确定了innexin2（inx2）的女性无菌等位基因，其编码果蝇中的间隙连接蛋白。在具有该inx2等位基因的雌性中，囊肿形成和卵腔形成受到损害。在野生型细菌中，Inx2在伴游细胞和卵泡细胞中强烈表达，两者都与种系细胞紧密接触。我们显示，胚芽体细胞中的inx2功能是早期生殖细胞的生存所必需的，并可能促进EGFR通路下游的囊肿形成，并且卵泡细胞中的inx2功能通过DE-钙黏着蛋白和火箭筒的调控促进卵腔形成（巴兹）在生殖细胞和卵泡细胞之间的边界。此外，遗传实验表明inx2与零种群增长（zpg）基因相互作用，该基因编码种系特异性间隙连接蛋白。这些结果表明Inx2间隙连接在体细胞支持细胞中在早期生殖细胞存活，囊肿形成和卵腔形成的调节中起着多功能作用。 Inx2间隙连接可能介导生殖细胞和体细胞支持细胞之间营养物质和信号分子的转移，并在调节细胞黏附中发挥作用。版权版权所有2011 Elsevier Ireland Ltd.保留所有权利。

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