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首页> 外文期刊>International journal of urology: official journal of the Japanese Urological Association >Oxalate-degrading Providencia rettgeri isolated from human stools.
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Oxalate-degrading Providencia rettgeri isolated from human stools.

机译：从人粪便中分离出降解草酸的普罗维登斯氏菌。

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BACKGROUND: Oxalate-degrading bacteria are thought to metabolize intestinal oxalate and thus decrease the urinary excretion of oxalate by reducing its intestinal absorption. METHODS: We have isolated several novel oxalate-degrading bacteria from human stools. Oxalate degrading bacteria were investigated to characterize their protein profiles with antibodies against oxalyl-coenzyme A decarboxylase (65 kDa) and formyl-coenzyme A transferase (48 kDa) purified from Oxalobacter formigenes. RESULTS: One of these isolates was identified as Providencia rettgeri, which showed two proteins (65 kDa and 48 kDa) on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) that were not found in non-oxalate-degrading P. rettgeri. Antibodies reacted with the 65 and 48 kDa proteins from the P. rettgeri strain on Western blotting. An Oxalobacter formigenes formyl-coenzyme A transferase gene probe reacted with chromosomal DNA from P. rettgeri on Southern blotting under high stringency conditions, while an Oxalobacter formigenes oxalyl-coenzyme A decarboxylase gene probe did not react under the same conditions. CONCLUSIONS: The mechamism of oxalate degradation by P. rettgeri appears to be similar to that of Oxalobacter formigenes. This is the first report of a facultative oxalate-degrading organism that is one of the Enterobacteriaceae.
机译：背景：草酸盐降解细菌被认为会代谢草酸肠，从而通过减少其肠道吸收来减少草酸的尿排泄。方法：我们从人粪便中分离出了几种新的可降解草酸盐的细菌。对草酸盐降解细菌进行了研究，以用抗草酰辅酶A脱羧酶（65 kDa）和甲酰辅酶A转移酶（48 kDa）从富氧草酸酶纯化的抗体表征其蛋白质谱。结果：这些分离株之一被鉴定为普罗维登斯氏菌，其在十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）上显示出两种蛋白质（65 kDa和48 kDa），这些蛋白质在非草酸盐降解瑞氏假单胞菌中未发现。在Western印迹中，抗体与瑞氏疟原虫菌株的65和48 kDa蛋白反应。在严格条件下，Southern印迹法检测到一种产甲烷氧化酶甲酰辅酶A转移酶基因探针与雷氏假单胞菌的染色体DNA进行了反应，而在相同条件下，产甲烷氧化酶草酰辅酶A脱羧酶基因探针未发生反应。结论：雷氏假单胞菌降解草酸盐的机理似乎与富氧草酸杆菌相似。这是一种可分解​​草酸盐的生物的首次报道，该生物是肠杆菌科的一种。

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