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首页> 外文期刊>Biochemistry >RELATIVE THERMODYNAMIC STABILITY OF DNA, RNA, AND DNA-RNA HYBRID DUPLEXES - RELATIONSHIP WITH BASE COMPOSITION AND STRUCTURE
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RELATIVE THERMODYNAMIC STABILITY OF DNA, RNA, AND DNA-RNA HYBRID DUPLEXES - RELATIONSHIP WITH BASE COMPOSITION AND STRUCTURE

机译：DNA，RNA和DNA-RNA混合双链体的相对热力学稳定性-与碱基组成和结构的关系

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摘要

Fourteen oligonucleotides 8-21 nucleotides in length and their complements were synthesized as DNA and RNA. For each sequence, four kinds of duplexes, DNA:DNA, RNA:RNA, DNA:RNA, and RNA:DNA, were prepared. Twelve sequences had A . T/U content varying from 25 to 80% and dPy content in the DNA strands varying from 0 to 100%. Thermodynamic stabilities of four duplexes for each sequence were determined in solution containing 100 mM Na+, 10 mM phosphate, and 0.1 mM EDTA, pH 7.1. CD spectra and electrophoretic mobility on native polyacrylamide gel were measured for most duplexes. Quantitative correlations of hybrid stability both with deoxypyrimidine content and, at fixed dPy content, with the fraction of A . T/U in duplexes were found. We also demonstrated that hybrids with 70-80% deoxypyrimidine DNA strand and a high or moderate A . T/U fraction displayed the highest relative stability compared to their RNA counterparts. Relationships of relative intensities of CD bands at 210 nm and relative electrophoretic mobilities of hybrids with relative hybrid stability suggested that hybrid conformation varies continuously between A- and B-form and is the decisive factor in relative hybrid stability.
机译：合成长度为14个8-21个核苷酸的寡核苷酸及其互补序列，作为DNA和RNA。对于每个序列，准备了四种双链体：DNA：DNA，RNA：RNA，DNA：RNA和RNA：DNA。十二个序列具有A。 T / U含量从25％到80％不等，DNA链中的dPy含量从0％到100％不等。在含有100 mM Na +，10 mM磷酸盐和0.1 mM EDTA，pH 7.1的溶液中确定每个序列的四个双链体的热力学稳定性。对于大多数双链体，测量了天然聚丙烯酰胺凝胶上的CD光谱和电泳迁移率。杂合稳定性与脱氧嘧啶含量以及在固定dPy含量下与A的分数之间的定量相关性。发现双工中的T / U。我们还证明了具有70-80％脱氧嘧啶DNA链和高或中度A的杂种。与RNA对应物相比，T / U分数显示出最高的相对稳定性。 210 nm的CD谱带的相对强度与杂交体的相对电泳迁移率与相对杂交体稳定性之间的关系表明，杂交体构象在A型和B型之间连续变化，并且是相对杂交体稳定性的决定性因素。

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