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首页> 外文期刊>Middle Eastern and Russian Journal of Plant Science and Biotechnology >RAPD and ISSR Markers Related to Drought Tolerance of Regenerated Plants in Wheat Double Haploids and Varieties
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RAPD and ISSR Markers Related to Drought Tolerance of Regenerated Plants in Wheat Double Haploids and Varieties

机译：与小麦双单倍体和新品种再生植物的耐旱性相关的RAPD和ISSR标记

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To verify the most drought-tolerant genotypes and to determine the appropriate concentration of polyethylene glycol PEG) that could be used in callus induction and plant regeneration, 10 double haploid (DH) genotypes and five varieties of bread wheatwere grown in the field under drought stress conditions and germinated in Petri dishes under 0, 10, 20 and 30% PEG. Five DH genotypes and two varieties (Giza 168 and Sakha 93) that were characterized by their high drought tolerant were selected.to, use:as sources of matare embryos to produce calli. the growing calli were transferred to callus culture medium with 0, 10 and 20% PEG 6000 to induce douugh stress for 30 days. Two of the five selected DH genotypes and the two wheat varieties induced enoughcalli and successfully regenerated under drought stress. RAPD analysis using three random primers showed that 28 of 39 total amplified fragments were polymorphic with 72.8% polymorphism under 10 and 20% PEG, whereas 91.7% polymorphism in primer B-09 washigher than that observed for the other two primers B-14 and C-15 (66.7 and 60%, respectively). The four wheat genotypes revealed 19 specific markers for drought tolerance with polymorphic mean percentage 50.6%. ISSR analysis using four different primersfor Brought tolerance revealed 35 polymorphic fragments with 57.3% mean polymorphism from a total of 61 amplified fragments under salinity stress^ The four wheat genotypes had 25 specific markers for drought tolerance with 41% mean polymorphism, Sakha 93 and DH-1 bad the highest total number of maekers at 10% PEG and Giza 168 at 20% PEG, while DH-1 had the highest total number of markers at the two PEG concentrations. RAPD and ISSR are significant methods to detect specific markers for drought tolerance and could be used in marker assisted selection (MAS) of wheat breeding programs to predict the most tolerant genotypes.
机译：为了验证最耐旱的基因型并确定可用于愈伤组织诱导和植物再生的聚乙二醇（PEG）浓度，在干旱胁迫下田间种植了10种双单倍体（DH）基因型和5个面包小麦品种并在0、10、20和30％PEG的陪替氏培养皿中发芽。选择了五个具有高耐旱性的DH基因型和两个变种（Giza 168和Sakha 93），用作：Matare胚的来源以产生愈伤组织。将生长的愈伤组织转移到含有0、10和20％PEG 6000的愈伤组织培养基中，以诱导30天的douugh胁迫。五个选定的DH基因型中的两个和两个小麦品种诱导足够的愈伤组织并在干旱胁迫下成功再生。使用三种随机引物的RAPD分析显示，在10％和20％PEG下，总共39个扩增片段中有28个是多态性，多态性为72.8％，而引物B-09中的91.7％多态性高于其他两个引物B-14和C。 -15（分别为66.7和60％）。四种小麦基因型揭示了19个抗旱性的特定标记，平均多态性百分比为50.6％。用四种不同引物进行的ISSR分析显示了在盐胁迫下总共61个扩增片段中有35个多态性片段，平均多态性为57.3％^四种小麦基因型具有25个抗旱性特异性标记，平均多态性为41％，Sakha 93和DH- 1在10％PEG和20％PEG的情况下，胶合剂的最高总数最高，而DH-1在两种PEG浓度下具有最高的标记总数。 RAPD和ISSR是检测抗旱性特定标记的重要方法，可用于小麦育种计划的标记辅助选择（MAS），以预测最能耐受的基因型。

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