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首页> 外文期刊>Middle Eastern and Russian Journal of Plant Science and Biotechnology >Antigenic Relationship between Two Bio-Agents and Pathogenic Fusarium semitectum Using Two Serological Techniques
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Antigenic Relationship between Two Bio-Agents and Pathogenic Fusarium semitectum Using Two Serological Techniques

机译：两种血清学技术检测两种生物制剂与半镰孢镰刀菌的抗原关系

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摘要

Wheat (Triticum aestivum L.) is an important crop cultivated successfully worldwide and in Egypt, which could be infected by Fusarium sp. Fungicides are generally used to control this pathogen. Nowadays, Trichoderma spp. and Pseudomonas fluorescens are used as biocontrol agents. In this study, a serological technique was carried out to explain the interaction between Trichoderma viride, P. fluorescens and the pathogenic fungus Fusarium semitectum using crossed-immunoelectrophoresis (CIE) and double diffusion reaction (DDR). In homologous reactions, the antigenic structures of F. semitectum, T. viride, and P. fluorescens were 7, 5 and 4 precipitin bands in CIE, while 2, 2, and 3 precipitin bands were detected in DDR, respectively. In heterologous reactions, antigens of T. viride electro-phoresed against antibodies of F. semitectum gave two common bands between them in either of the used methods, while P. fluorescens antigens gave only one common precipitin antigen when electrophoresed against antibodies of the pathogen using both methods. This clearly indicated that T. viride was able to recognize to the pathogenic fungus F. semitectum, which showed more common antigens.
机译：小麦（Triticum aestivum L.）是一种在世界范围内和埃及成功栽培的重要农作物，可能会被镰刀菌感染。杀菌剂通常用于控制这种病原体。如今，木霉属。荧光假单胞菌和荧光假单胞菌用作生物防治剂。在这项研究中，通过交叉免疫电泳（CIE）和双扩散反应（DDR），采用血清学技术解释了绿色木霉，荧光假单胞菌和致病性真菌半镰孢之间的相互作用。在同源反应中，半裸镰刀菌，T。viride和荧光假单胞菌的抗原结构在CIE中分别为7、5和4个沉淀蛋白条带，而在DDR中分别检测到2、2和3个沉淀蛋白条带。在异源反应中，用两种方法中的任一种用电泳法检测的半透明镰刀菌抗原在它们之间有两个共同的条带，而荧光假单胞菌抗原在使用病原体抗体进行电泳时仅给出一种共同的沉淀素抗原。两种方法。这清楚地表明，病毒绿假单胞菌能够识别病原性真菌半裸镰刀菌，后者显示出更多的常见抗原。

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