...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Military Medicine: Official Journal of AMSUS, The Society of the Federal Health Agencies >A rabbit osteomyelitis model to simulate multibacterial War Wound Infections
【24h】

A rabbit osteomyelitis model to simulate multibacterial War Wound Infections

机译：模拟多细菌战伤感染的兔骨髓炎模型

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A challenge facing militarycaregiversis thepresenceof multidrug-resistant infection inextremity wounds. Most frequently identifiedresistant strains are methicillin-resistant Staphylococcus aureus (MRSA), Klebsiellapneumoniae (KP), Pseudomonas aeruginosa (PA), and Acinetobacter baumannii (AB). We adapted an existing osteomyelitis model to simulate an infected extremity wound for antibiotic testing. New Zealand White Rabbits (n = 95) were divided into 6 inoculation groups for infection with MRSA, KP, PA, and AB alone, and in multibacteria infections. Sodium morrhuate was injected into the left tibia to simulate blast wound trauma, then the respective bacteria or combination of patho-gens, and finally sterile saline were injected. Colony-forming units for the mono-organism groups showed that AB, KP, or PA alone at 107colony-forming units per mL (CFUs/mL) was effective for rabbit osteomyelitis induction. Colony-forming units for the multiorganism groups showed that the combination of AB (107CFUs/mL)/KP (107CFUs/mL)/PA (107CFUs/mL)/MRSA (105CFUs/mL) yielded a 100% osteomyelitis induction rate. At 8 weeks, however, only one mono-bacterial group and one multibacterial group showed significant radiographic improvement (p 0.05). The rabbit model of osteomyelitis can be adapted to study infected blast wounds typical of those seen in veterans. To our knowledge, this is the first demonstration of the model simulating multibacterial infections with multidrug-resistant organisms.
机译：军事护理人员面临的挑战是存在多种药物感染的肢体伤口。最常见的耐药菌株是耐甲氧西林的金黄色葡萄球菌（MRSA），肺炎克雷伯氏菌（KP），铜绿假单胞菌（PA）和鲍曼不动杆菌（AB）。我们改编了现有的骨髓炎模型，以模拟感染肢体伤口进行抗生素测试。将新西兰白兔（n = 95）分为6个接种组，分别接受MRSA，KP，PA和AB感染，以及多细菌感染。向左胫骨注射促渗钠以模拟爆炸伤口创伤，然后分别注射细菌或病原体，并注射无菌盐水。单菌群的菌落形成单位显示，单独的AB，KP或PA的菌落形成单位为107 mL / mL（CFUs / mL）对诱导兔骨髓炎有效。微生物群的菌落形成单位显示，AB（107CFUs / mL）/ KP（107CFUs / mL）/ PA（107CFUs / mL）/ MRSA（105CFUs / mL）的组合产生了100％的骨髓炎诱导率。然而，在8周时，只有一个单细菌组和一个多细菌组显示出显着的影像学改善（p <0.05）。骨髓炎的兔子模型可以适应研究退伍军人中典型的受感染的爆炸伤口。据我们所知，这是该模型对具有多药耐药菌的多细菌感染进行模拟的首次演示。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号