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首页> 外文期刊>Biochemistry >TRANSBILAYER TRANSPORT OF IONS AND LIPIDS COUPLED WITH MASTOPARAN X TRANSLOCATION
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TRANSBILAYER TRANSPORT OF IONS AND LIPIDS COUPLED WITH MASTOPARAN X TRANSLOCATION

机译：MASTOPARAN X转运耦合的离子和脂质的转运体运输

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摘要

The transbilayer movement of ions and lipids induced by mastoparan X, a peptidic toxin from Vespa xanthoptera, was investigated by use of lipid vesicles asa model membrane system. Negatively charged phosphatidylglycerol remarkably enhanced the peptide-lipid interactions. Mastoparan X induced the ion flow by forming a short-lived, multimeric pore in the lipid bilayer, as determined from the leakage of an anionic dye, calcein, from the liposomes. The pore formation was coupled with the translocation of the peptide into the inner leaflet. The latter was detected by three experiments using fluorescence techniques [Matsuzaki, K., Murase, O., Fujii, N., & Miyajima, K. (1995) Biochemistry 34, 6521-6526; Matsuzaki, K., Murase, O., Br Miyajima, K. (1995) Biochemistry 34, 12553-12559]. The lipid flip flop was monitored on the basis of the chemical quenching of 7-nitrobenz-2-oxa-1-3,diazol-4-yl (NBD)-labeled lipids by sodium dithionite. Mastoparan X triggered the rapid flip-flop of both negatively charged and zwitterionic lipids in coupling with the pore formation and the peptide translocation. A novel model of the mastoparan-lipid interactions was proposed to explain these observations.
机译：通过使用脂质囊泡作为模型膜系统研究了马索帕兰X（一种来自黄蜂类黄蜂的肽毒素）诱导的离子和脂质的跨层运动。带负电的磷脂酰甘油显着增强了肽-脂质相互作用。 Mastoparan X通过在脂质双层中形成一个短寿命的多聚体孔来诱导离子流，这是由阴离子染料钙黄绿素从脂质体中泄漏所确定的。孔的形成与肽易位到内部小叶中有关。后者通过使用荧光技术的三个实验来检测[Matsuzaki，K.，Murase，O.，Fujii，N。，和Miyajima，K。（1995）Biochemistry 34，6521-6526; M.M.，Chem.Soc。，1993，5，1897]。 Matsuzaki，K.，Murase，O.，Br Miyajima，K。（1995）Biochemistry 34，12553-12559]。基于连二亚硫酸钠对7-硝基苯-2-氧杂-1-3，二氮杂-4-基（NBD）标记的脂质的化学淬灭来监测脂质触发器。 Mastoparan X触发了带负电荷的脂质和两性离子脂质的快速翻转，并与孔形成和肽易位结合。提出了一种新的模型的乳脂素-脂质相互作用来解释这些观察结果。

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