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首页> 外文期刊>Biochemistry >Biologically Active Oligosaccharides from Pectins of Pisum sativum L. Seedlings Affecting Root Generation
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Biologically Active Oligosaccharides from Pectins of Pisum sativum L. Seedlings Affecting Root Generation

机译：豌豆幼苗果胶中生物活性低聚糖对根系生成的影响

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摘要

Two physiologically active oligosaccharide fractions were isolated from pectin of Pisum sativum L. cell wall after its partial acid hydrolysis. These fractions displayed stimulating and inhibiting effects on root formation in thin-layer explants. The subsequent separation of these fractions by gel permeation and anion-exchange chromatography resulted in fractions with effective concentrations two orders of magnitude lower than the concentrations of the initial fractions. The resulting oligosaccharides displayed their effect on the earliest stage of the rhizogenesis associated with formation of root primordias. The rhizogenesis-inhibiting fraction suppressed cell division by 30-50%. The stimulating fraction mainly contained fragments of xyloglucan and galactan, and the inhibiting fraction contained fragments of xyloglucan, glactan, and arabinan. The polymerization degrees of the stimulating and of the inhibiting oligosaccharides were 10-11 and 5-6, respetively.
机译：在部分酸水解后，从Pisum sativum L.细胞壁的果胶中分离出两个具有生理活性的低聚糖级分。这些级分显示出对薄层外植体中根形成的刺激和抑制作用。随后通过凝胶渗透和阴离子交换色谱分离这些馏分，得到的馏分的有效浓度比初始馏分的浓度低两个数量级。所产生的寡糖在与根原基形成相关的发根的最早阶段显示出其作用。根瘤抑制组分抑制细胞分裂30-50％。刺激级分主要包含木葡聚糖和半乳聚糖的片段，而抑制级分包含木葡聚糖，甘露聚糖和阿拉伯聚糖的片段。刺激性寡糖和抑制性寡糖的聚合度分别为10-11和5-6。

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