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首页> 外文期刊>F.O. Licht's International Sugar & Sweetener Report >Identification of (R)-2-butanol as a sex attractant pheromone of the white grub beetle, Dasylepida ishigakiensis (Coleoptera: Scarabaeidae), a serious sugarcane pest in the Miyako Islands of Japan
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Identification of (R)-2-butanol as a sex attractant pheromone of the white grub beetle, Dasylepida ishigakiensis (Coleoptera: Scarabaeidae), a serious sugarcane pest in the Miyako Islands of Japan

机译：鉴定（R）-2-丁醇为白色Da甲虫（日本鳞翅目（Soleabaeidae）的一种白色g甲虫）的性诱剂信息素，这是日本宫古群岛的一种严重甘蔗害虫

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The white grub beetle, Dasylepida ishigakiensis (Niijima et Kinoshita), is a serious pest of sugarcane in the Miyako Islands of Okinawa, Japan. Two evident electroantennographic (EAG) responses were observed by solid-phase microextraction sampling from female secretion and subsequent gas chromatography-electroantennographic detection (GC-EAD) analyses. These were identified as 2-propanol and 2-butanol by GC-MS analyses. GC-EAD on a chiral column showed two evident EAG signals corresponding to 2-butanol enantiomers, but the ratio and amounts could not be determined because of the small amounts. (R)-2-Butanol elicited the strongest EAG response, followed by (S)-2-butanol and 2-propanol, which showed 1/10 and 1/100 of the response of the first compound, respectively. In the field, only (R)-2-butanol attracted feral males when it was baited as 1 ml water solution of 1-100 mu g of this compound, (S)2-butanol, racemic mixture or 2-propanol on cotton ball lures (ca. 1.5 cm diameter) in vane-traps. When 2-propanol was blended with 10 mu g/ml of (R)-2-butanol solution on cotton ball lures, male catches decreased as the amount of 2-propanol increased. From these results it was concluded that (R)-2-butanol was a component of the sex attractant pheromone of D. ishigakiensis while (S)-enantiomer and 2-propanol showed inhibitory effects when they were blended with the first compound.
机译：白色g甲虫Dasylepida ishigakiensis（Niijima et Kinoshita）是日本冲绳县宫古岛的一种严重的甘蔗害虫。通过从雌性分泌物进行固相微萃取采样以及随后的气相色谱-电子血管造影检测（GC-EAD）分析，观察到两个明显的电子血管造影（EAG）反应。通过GC-MS分析鉴定为2-丙醇和2-丁醇。手性柱上的GC-EAD显示出两个明显的EAG信号，分别对应于2-丁醇对映体，但由于量少，无法确定其比例和含量。 （R）-2-丁醇引起最强的EAG响应，其次是（S）-2-丁醇和2-丙醇，分别显示出第一种化合物响应的1/10和1/100。在田间，当将（R）-2-丁醇以1 ml 1-100μg该化合物，（S）2-丁醇，外消旋混合物或2-丙醇的水溶液引诱到棉球上时，仅能吸引野生雄性。诱集诱饵（直径约1.5厘米）。当将2-丙醇与10μg / ml的（R）-2-丁醇溶液在棉球诱饵上混合时，随着2-丙醇量的增加，雄性捕获量减少。从这些结果可以得出结论，（R）-2-丁醇是石垣D.性引诱剂信息素的成分，而（S）-对映体和2-丙醇与第一种化合物混合时则表现出抑制作用。

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