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首页> 外文期刊>Mitochondrial DNA >Identification of goat cashmere and sheep wool by PCR-RFLP analysis of mitochondrial 12S rRNA gene
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Identification of goat cashmere and sheep wool by PCR-RFLP analysis of mitochondrial 12S rRNA gene

机译：线粒体12S rRNA基因的PCR-RFLP分析鉴定山羊绒和绵羊毛

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摘要

The efficacy of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of mitochondrial 12S rRNA gene in identification of goat cashmere and sheep wool samples was evaluated. The specific fragments of the mitochondrial 12S rRNA gene, which were about 440 bp, were obtained using the PCR. Restriction enzyme digestion of the PCR products with endonucleases BspT I and Hinf I revealed species-specific RFLP patterns. Application of this technique on mixed samples could identify goat cashmere and sheep wool from each other within the proportion of 8:1. The technique, however, could detect only one species when the proportion of mixture was more than 9:1. The PCR-RFLP technique was demonstrated to possess potential value in precise identification of goat cashmere and sheep wool.
机译：评估了线粒体12S rRNA基因的聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析在鉴定山羊绒和绵羊羊毛样品中的功效。使用PCR获得了约440bp的线粒体12SrRNA基因的特异性片段。用核酸内切酶BspT I和Hinf I对PCR产物进行限制性酶切，揭示了物种特异性RFLP模式。该技术在混合样品上的应用可以在8：1的比例下彼此鉴别山羊绒和羊绒。但是，当混合物的比例超过9：1时，该技术只能检测一种物种。 PCR-RFLP技术被证明具有精确鉴定山羊绒和绵羊绒的潜在价值。

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