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首页> 外文期刊>Mitochondrial DNA, Part A >Impacts of low temperature preservation on mitochondrial DNA copy number in oocytes of the hard coral Echinopora sp.
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Impacts of low temperature preservation on mitochondrial DNA copy number in oocytes of the hard coral Echinopora sp.

机译：低温保存对硬珊瑚棘皮鱼卵母细胞线粒体DNA拷贝数的影响。

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摘要

Given the current threats to coral reefs worldwide, there is an urgent need to develop protocols for the cryopreservation of reef-building corals. However, chilling may alter coral mitochondrial distribution and membrane potential, resulting in reduced ATP production. The aim of this study was to investigate the impacts of chilling on mitochondrial DNA copy number (CN) in oocytes of the hard coral Echinopora sp. Oocytes were exposed to 0.5 M, 1 M or 2 M methanol at 5, 0 or -5 °C for 2,4, 8 and 16 h. When oocytes were chilled with no cryoprotectant (CPT) or 1 M methanol at 5 or 0°C, the mtDNA CNs initially increased at hour 2 of incubation, although it decreased significantly over the 16 h of incubation in chilled oocytes at -5°C. The mtDNA CN increased and picked in 0.5 M methanol at 5°C and 0°C at hour 8 of incubation in chilled oocytes indicating that the high mtDNA CN of these oocytes is probably responsible for withstanding high chilling sensitivity. We currently propose that 0.5 M methanol is the optimal CPT for oocytes of Echinopora sp., and potentially other reef corals.
机译：鉴于目前全球范围内对珊瑚礁的威胁，迫切需要制定用于冷冻保存珊瑚礁的珊瑚的方案。但是，冷却可能会改变珊瑚线粒体的分布和膜电位，从而导致ATP产量降低。这项研究的目的是调查冷对硬珊瑚Echinopora sp卵母细胞线粒体DNA拷贝数（CN）的影响。将卵母细胞在5、0或-5°C下暴露于0.5 M，1 M或2 M甲醇中2,4、8和16 h。当卵母细胞在5°C或0°C下没有冷冻保护剂（CPT）或1 M甲醇冷却时，虽然在-5°C的冷冻卵母细胞培养16小时后，其mtDNA CNs在培养的第2小时开始显着降低，但其最初的增加。在冷藏卵母细胞中孵育的第8小时，mtDNA CN升高并在0.5°C和0°C的0.5 M甲醇中被挑选出来，表明这些卵母细胞的高mtDNA CN可能是承受高冷却敏感性的原因。我们目前建议0.5 M甲醇是Echinopora sp。和其他潜在珊瑚礁卵母细胞的最佳CPT。

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