A simple method to design PCR primer to detect genomic DNA of parasites and its application to Dirofilaria immitis.

Nagano I; Zhiliang W; Nakayama M; Takahashi Y

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A simple method to design PCR primer to detect genomic DNA of parasites and its application to Dirofilaria immitis.

机译：一种设计PCR引物检测寄生虫基因组DNA的简单方法及其在Dirofilaria炎中的应用。

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A simple method to design a polymerase chain reaction (PCR) primer for parasites of interest was developed using Dirofilaria immitis as a test sample. The method involved the cloning and sequencing of randomly amplified DNA of the parasite, and designing a primer based on the resulting DNA sequence. Using the primer, DNA fragments of the expected length were amplified by a regular PCR with genomic DNA of Dirofilaria immitis.

机译：设计了一种简单的方法来设计感兴趣的寄生虫的聚合酶链反应（PCR）引物，方法是使用Dirofilaria免疫炎作为测试样品。该方法涉及对寄生虫的随机扩增DNA进行克隆和测序，并根据所得DNA序列设计引物。使用引物，通过常规PCR，用Dirofilaria炎的基因组DNA扩增预期长度的DNA片段。

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《Molecular and Cellular Probes: The Location, Diagnosis and Monitoring of Disease by Specific Molecules and Cell Lines》 |1996年第6期|共3页
作者
Nagano I; Zhiliang W; Nakayama M; Takahashi Y;
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原文格式 PDF
正文语种 eng
中图分类基础医学;
关键词
DNA Primers; DNA; Helminth; Dirofilaria immitis; Polymerase Chain Reaction; DNA引物; DNA; 蠕虫; 恶丝虫; 犬; 聚合酶链反应;

机译：DNA Primers;DNA;Helminth;Dirofilaria immitis;Polymerase Chain Reaction;DNA引物;DNA;蠕虫;恶丝虫;犬;聚合酶链反应;

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1. A simple method to design PCR primer to detect genomic DNA of parasites and its application to Dirofilaria immitis. [J] . Nagano I, Zhiliang W, Nakayama M, Molecular and Cellular Probes: The Location, Diagnosis and Monitoring of Disease by Specific Molecules and Cell Lines . 1996,第6期

机译：一种设计PCR引物检测寄生虫基因组DNA的简单方法及其在Dirofilaria炎中的应用。
2. Orthologues of the Drosophila melanogaster E75 molting control gene in the filarial parasites Brugia malayi and Dirofilaria immitis. [J] . Crossgrove K, Maina CV, Robinson-Rechavi M, Molecular and Biochemical Parasitology . 2008,第1期

机译：果蝇E75蜕皮控制基因的直向同源物在丝状寄生虫马来亚布鲁氏菌和Dirofilaria免疫炎中。
3. Removal of hydrogen peroxide by a 1-cysteine peroxiredoxin enzyme of the filarial parasite Dirofilaria immitis. [J] . Chandrashekar R, Tsuji N, Morales TH, Parasitology Research . 2000,第3期

机译：丝状寄生虫Dirofilaria炎症的1-半胱氨酸过氧化物酶去除过氧化氢。
4. A Simple and rapid method for preparation of Rhizopus Oryzae genomic DNA for PCR analysis [C] . Xinhui Wang, Yin Zhang, Wei Wang Annual International Meeting of the American Society of Agricultural and Biological Engineers . 2014

机译：制备Rhizopus Oryzae基因组DNA的简单且快速的方法，用于PCR分析
5. Design and Implementation of Degenerate qPCR/qRT-PCR Primers to Detect Microbial Nitrogen Metabolism in Wastewater and Wastewater-Related Samples [D] . Keeley, Ryan F. 2019

机译：退化QPCR / QRT-PCR引物的设计与实现，以检测废水和废水相关样品中的微生物氮代谢
6. Optimal RNA isolation method and primer design to detect gene knockdown by qPCR when validating Drosophila transgenic RNAi lines [O] . Roslyn L. Mainland, Taylor A. Lyons, Mike M. Ruth, 2017

机译：验证果蝇转基因RNAi品系时通过qPCR检测基因敲低的最佳RNA分离方法和引物设计
7. Cloning of a cuticular antigen that contains multiple tandem repeats from the filarial parasite Dirofilaria immitis. [O] . Poole, C B, Grandea, A G, Maina, C V, 1992

机译：克隆含有丝状寄生虫Dirofilaria免疫炎的多个串联重复序列的表皮抗原。

A simple method to design PCR primer to detect genomic DNA of parasites and its application to Dirofilaria immitis.

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