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首页> 外文期刊>Molecular Biology >Ameliorative effect of curcumin on aflatoxin B1-induced changes in liver gene expression of Oreochromis niloticus
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Ameliorative effect of curcumin on aflatoxin B1-induced changes in liver gene expression of Oreochromis niloticus

机译：姜黄素对黄曲霉毒素B1诱导的尼罗罗非鱼肝基因表达变化的改善作用

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摘要

To evaluate the ameliorative effect of curcumin on dietary aflatoxin-induced changes in the expression of genes in Nile tilapia Oreochromis niloticus liver, the fish were fed with a diet contaminated by 200 ppb of aflatoxin B1 (AFB1) with and without curcumin (5 mg/kg diet) for 16 weeks in addition to a negative and positive controls fed with the basal diet and basal diet supplemented with curcumin, respectively. Further, two recovery groups with and without curcumin were tested after 2 more weeks. Relative mRNA expression of genes involved in antioxidant function (superoxide dismutase, SOD), biotransformation (cytochrome P4501A, CYP1A) and immune response (interleukin-1 beta, IL-1 beta and transforming growth factor beta, TGF-beta) were assessed using RT-PCR. Also, fish weight gain and survival rate were determined. Results revealed that AFB1 significantly reduced the survivability and weight gain, while curcumin inclusion improved them. Fish fed with AFB1-contaminated diet showed the up-regulation of CYP1A and down-regulation of SOD, IL-1 beta and TGF-beta. This expression pattern was still evident in the recovery group without curcumin, but to a lesser extent. Supplementation of curcumin ameliorated the overall gene expression close to the control levels. It appears that curcumin exhibited protective effects on AFB1-induced liver toxicity in O. niloticus by moderating oxidative stress, toxin biotransformation, immune response, and hence growth performance.
机译：为了评估姜黄素对日粮黄曲霉毒素引起的尼罗罗非鱼Oreochromis niloticus肝脏中基因表达变化的改善作用，给鱼饲喂受有200 ppb黄曲霉毒素B1（AFB1）污染的饮食，其中有或没有姜黄素（5 mg /分别以基础饮食和补充姜黄素的基础饮食喂养的阴性和阳性对照者，分别进行16周的饮食。此外，在另外2周后测试了有和没有姜黄素的两个恢复组。使用RT评估涉及抗氧化功能（超氧化物歧化酶，SOD），生物转化（细胞色素P4501A，CYP1A）和免疫应答（白介素-1 beta，IL-1 beta和转化生长因子beta，TGF-beta）的基因的相对mRNA表达。 -PCR。另外，确定鱼的体重增加和成活率。结果显示，AFB1显着降低了生存能力和体重增加，而姜黄素的包合改善了生存能力和体重增加。用AFB1污染的饮食喂养的鱼显示CYP1A上调，而SOD，IL-1β和TGF-β则下调。在没有姜黄素的恢复组中，这种表达模式仍然很明显，但程度较小。补充姜黄素可改善总体基因表达，使其接近对照水平。姜黄素似乎通过抑制氧化应激，毒素生物转化，免疫反应以及由此而来的生长表现出对尼罗罗非鱼AFB1诱导的肝毒性的保护作用。

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