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首页> 外文期刊>Molecular biology and evolution >Identification of RNA editing sites in chloroplast transcripts from the maternal and paternal progenitors of tobacco (Nicotiana tabacum): Comparative analysis shows the involvement of distinct trans-factors for ndhB editing
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Identification of RNA editing sites in chloroplast transcripts from the maternal and paternal progenitors of tobacco (Nicotiana tabacum): Comparative analysis shows the involvement of distinct trans-factors for ndhB editing

机译：烟草（Nicotiana tabacum）母代和父代叶绿体转录本中RNA编辑位点的鉴定：对比分析显示了不同的反式因子参与ndhB编辑

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摘要

RNA editing alters genomic nucleotide sequences at the transcript level. In higher plant chloroplasts, C-to-U conversion is known to occur at around 30 specific sites. The tobacco cultivar Nicotiana tabacum is an amphidiploid derived from ancestors of N. sylvestris (maternal) and N. tomentosiformis (paternal). The chloroplast genome of N. tabacum is believed to originate from an ancestor of N. sylvestris. To study the evolution of RNA editing in higher plant chloroplasts, editing sites in the two likely progenitors have first been identified based on those found in N. tabacum. Altogether 34, 33, and 32 editing sites have been found in the chloroplast transcripts from N. tabacum, N. sylvestris, and N. tomentosiformis, respectively. Thirty-one sites are conserved among the three species, whereas remarkable differences are observed in the editing of ndhB and ndhD transcripts. Sites 7 and 8 in ndhB mRNAs are separated only by five nt, and both are edited in N. tabacum and N. sylvestris. However, site 8 is not edited in N. tomentosiformis, indicating that distinct trans-factors are involved in the two editing events. The first site in ndhD mRNAs is edited to produce an AUG start codon in N. sylvestris as well as in N. tabacum but not in N. tomentosiformis, suggesting that a distinct mechanism operates for the translational initiation of N. tomentosiformis ndhD mRNAs. Four to six sites are edited partially in green leaves. Some of these sites may represent evolutionary intermediates in the process of losing editing events.
机译：RNA编辑可在转录水平上改变基因组核苷酸序列。在高等植物的叶绿体中，已知C到U的转化发生在大约30个特定位点。烟草栽培种烟草Nicotiana tabacum是一种二倍体，衍生自樟子松猪笼草（母本）和绒毛猪笼草（父本）的祖先。据信烟草的叶绿体基因组起源于樟子松的祖先。为了研究高等植物叶绿体中RNA编辑的进化，首先基于烟草中发现的两个可能的祖细胞中的编辑位点进行了鉴定。分别在来自烟草，烟草和毛状烟草的叶绿体转录物中共发现了34、33和32个编辑位点。在这三个物种中，有三十一个位点是保守的，而在ndhB和ndhD转录本的编辑中观察到显着差异。 ndhB mRNA中的位点7和8仅被五个nt分隔，并且都在烟草和樟脑中进行编辑。但是，位点8没有在绒毛猪笼草中编辑，这表明在两个编辑事件中涉及不同的反式因子。编辑了ndhD mRNA的第一个位点，以在樟脑猪笼草和烟草中产生AUG起始密码子，而在绒毛猪笼草中则没有，这提示了一种不同的机制可用于翻译棉铃虫ndhD mRNA。四个到六个站点以绿叶部分编辑。这些站点中的某些站点可能代表丢失编辑事件过程中的进化中间产物。

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